0.01M TBS (pH7.4) with 1% rAlbumin, 0.02% Proclin300 and 50% Glycerol.
Formulierung:
Liquid
Target-Kategorie:
KCNJ2
Application Verdünnung:
WB=1:500-2000, Flow-Cyt=1µg/Test
Flow cytometric analysis of Rsc96 cell using Kir2.1 antibody.
Western blot analysis of extracts from Heart tissue(mo) using Kir2.1 antibody.
Blank control (blue): RSC96 (fixed with 2% paraformaldehyde (10 min)). Primary Antibody: Rabbit Anti-Kir2.1 antibody (orb184788), dilution: 0.2 µg in 100 µl 1X PBS containing 0.5% BSA, Isotype Control Antibody: Rabbit IgG (orange), used under the same conditions, Secondary Antibody: Goat anti-rabbit IgG-PE (white blue), dilution: 1:200 in 1X PBS containing 0.5% BSA.
Sample: Bone (Mouse) Lysate at 40 ug, Primary: Anti-Kir2.1 at 1/300 dilution, Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution, Predicted band size: 48 kD, Observed band size: 48 kD.
Sample: heart (Mouse) Lysate at 40 ug, Primary: Anti-Kir2.1 (orb184788) at 1/300 dilution, Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution, Predicted band size: 48 kD, Observed band size: 48 kD.
Sample: Muscle (Mouse) Lysate at 40 ug, Primary: Anti-Kir2.1 at 1/300 dilution, Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution, Predicted band size: 48 kD, Observed band size: 48 kD.
* Mehrwertsteuer und Versandkosten nicht enthalten. Irrtümer und Preisänderungen vorbehalten
