KCNV1 Rabbit Polyclonal Antibody, Unconjugated

Artikelnummer: BYT-ORB184651
Artikelname: KCNV1 Rabbit Polyclonal Antibody, Unconjugated
Artikelnummer: BYT-ORB184651
Hersteller Artikelnummer: orb184651
Alternativnummer: BYT-ORB184651-50,BYT-ORB184651-100,BYT-ORB184651-200
Hersteller: Biorbyt
Wirt: Rabbit
Kategorie: Antikörper
Applikation: IF, IHC-Fr, IHC-P, WB
Spezies Reaktivität: Mouse, Rat
Immunogen: KLH conjugated synthetic peptide derived from human KCNT1 (251-350/500aa)
Konjugation: Unconjugated
Alternative Synonym: HNKA, KCNB3, KV2.3, KV8.1, 2700023A03Rik, vibe, kv2.3r, KCNV1_BOVIN, KCNV1, Voltage-gated potassium channel subunit Kv8.1, KCNV1_HUMAN, Neuronal potassium channel alpha subunit HNKA, KCNV1_MOUSE, KCNV1_RAT, Voltage-gated potassium channel subunit Kv2.3r,
KCNV1 Rabbit Polyclonal Antibody
Klonalität: Polyclonal
Konzentration: 1mg/ml
Molekulargewicht: 56 kDa
UniProt: Q6PIU1
Puffer: 0.01M TBS (pH7.4) with 1% rAlbumin, 0.02% Proclin300 and 50% Glycerol.
Formulierung: Liquid
Target-Kategorie: KCNV1
Application Verdünnung: WB=1:500-2000, IHC-P=1:100-500, IHC-F=1:100-500, IF=1:100-500
Immunohistochemical staining of Rat kidney tissue using KCNV1 antibody.
Immunohistochemical staining of rat brain tissue using KCNV1 antibody.
Paraformaldehyde-fixed, paraffin embedded (Rat brain), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37C for 30 min, Antibody incubation with (KCNV1) Polyclonal Antibody, Unconjugated (orb184651) at 1:400 overnight at 4C, followed by a conjugated secondary for 20 minutes and DAB staining.
Sample: Cerebrum (Mouse) Lysate at 40 ug, Primary: Anti-KCNV1 (orb184651) at 1/300 dilution, Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution, Predicted band size: 56 kD, Observed band size: 56 kD.
Sample: Lane 1: Mouse Cerebrum tissue lysates, Lane 2: Rat Cerebrum tissue lysates, Primary: Anti-KCNV1 (orb184651) at 1/1000 dilution, Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution, Predicted band size: 56 kDa, Observed band size: 46 kDa.
Tissue/Cell: Rat kidney tissue, 4% Paraformaldehyde-fixed and paraffin-embedded, Antigen retrieval: citrate buffer (0.01M, pH 6.0), Boiling bathing for 15 min, Block endogenous peroxidase by 3% Hydrogen peroxide for 30 min, Blocking buffer (normal goat serum) at 37C for 20 min, Incubation: Anti-KCNV1 Polyclonal Antibody, Unconjugated (orb184651) 1:200, overnight at 4C, followed by conjugation to the secondary antibody and DAB staining.



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