GLUT2 Rabbit Polyclonal Antibody, Unconjugated

Artikelnummer: BYT-ORB183810
Artikelname: GLUT2 Rabbit Polyclonal Antibody, Unconjugated
Artikelnummer: BYT-ORB183810
Hersteller Artikelnummer: orb183810
Alternativnummer: BYT-ORB183810-50,BYT-ORB183810-100,BYT-ORB183810-200
Hersteller: Biorbyt
Wirt: Rabbit
Kategorie: Antikörper
Applikation: FC, IF, IHC-Fr, IHC-P, WB
Spezies Reaktivität: Human, Mouse, Rat
Immunogen: KLH conjugated synthetic peptide derived from human GLUT2 (1-100/524aa)
Konjugation: Unconjugated
Alternative Synonym: GTR2_HUMAN, SLC2A2, Glucose transporter type 2, liver (GLUT-2), GLUT2, GTR2_MOUSE, GTR2_RAT,
GLUT2 Rabbit Polyclonal Antibody
Klonalität: Polyclonal
Konzentration: 1mg/ml
Molekulargewicht: 54 kDa
UniProt: P11168
Puffer: 0.01M TBS (pH7.4) with 1% rAlbumin, 0.02% Proclin300 and 50% Glycerol.
Formulierung: Liquid
Target-Kategorie: SLC2A2
Application Verdünnung: WB=1:500-2000, IHC-P=1:100-500, IHC-F=1:100-500, IF=1:100-500, Flow-Cyt=1µg/Test
Immunohistochemical staining of rat kidney tissue using GLUT2 antibody.
Blank control (blue line): Hep G2 (fixed with 70% ethanol Overnight at 4C). Primary Antibody (green line): Rabbit Anti-GLUT2 antibody (orb183810), dilution: 1 µg/10 6 cells, Isotype Control Antibody (orange line): Rabbit IgG. Secondary Antibody (white blue line): Goat anti-rabbit IgG-PE, dilution: 1 µg/Test.
Sample: Kidney (Mouse) Lysate at 40 ug, Primary: Anti-GLUT2 (orb183810) at 1/300 dilution, Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution, Predicted band size: 54 kD, Observed band size: 54 kD.
Sample: Lane 1: Liver (Mouse) Lysate at 40 ug, Lane 2: Kidney (Mouse) Lysate at 40 ug, Lane 3: Pancreas (Mouse) Lysate at 40 ug, Primary: Anti-GLUT2 (orb183810) at 1/1000 dilution, Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution, Predicted band size: 57/45 kD, Observed band size: 47 kD.
Sample: Liver (Mouse) Lysate at 40 ug, Primary: Anti-GLUT2 (orb183810) at 1/300 dilution, Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution, Predicted band size: 54 kD, Observed band size: 54 kD.
Tissue/Cell: rat kidney tissue, 4% Paraformaldehyde-fixed and paraffin-embedded, Antigen retrieval: citrate buffer (0.01M, pH 6.0), Boiling bathing for 15 min, Block endogenous peroxidase by 3% Hydrogen peroxide for 30 min, Blocking buffer (normal goat serum) at 37C for 20 min, Incubation: Anti-GLUT2 Polyclonal Antibody, Unconjugated (orb183810) 1:200, overnight at 4C, followed by conjugation to the secondary antibody and DAB staining.



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