CRHR1 Rabbit Polyclonal Antibody, Unconjugated

Artikelnummer: BYT-ORB182913
Artikelname: CRHR1 Rabbit Polyclonal Antibody, Unconjugated
Artikelnummer: BYT-ORB182913
Hersteller Artikelnummer: orb182913
Alternativnummer: BYT-ORB182913-50,BYT-ORB182913-100,BYT-ORB182913-200
Hersteller: Biorbyt
Wirt: Rabbit
Kategorie: Antikörper
Applikation: IF, IHC-Fr, IHC-P, WB
Spezies Reaktivität: Mouse, Rat
Immunogen: KLH conjugated synthetic peptide derived from human CRHR1 (55-150/444aa)
Konjugation: Unconjugated
Alternative Synonym: CRF-R, CRF-R-1, CRF-R1, CRF1, CRFR-1, CRFR1, CRH-R-1, CRH-R1, CRHR, CRHR1L, CRF-R1alpha, CRF1R, CRH-R, CRH-R 1, CRFR1_HUMAN, CRHR1, Corticotropin-releasing hormone receptor 1 (CRH-R-1 | CRH-R1), CRFR, CRFR1_MOUSE, CRFR1_RAT,
CRHR1 Rabbit Polyclonal Antibody
Klonalität: Polyclonal
Konzentration: 1mg/ml
Molekulargewicht: 48 kDa
UniProt: P34998
Puffer: 0.01M TBS (pH7.4) with 1% rAlbumin, 0.02% Proclin300 and 50% Glycerol.
Formulierung: Liquid
Target-Kategorie: CRHR1
Application Verdünnung: WB=1:500-2000, IHC-P=1:100-500, IHC-F=1:100-500, IF=1:100-500
Flow cytometric analysis of Rsc96 cell using CRHR1 antibody.
Immunohistochemical staining of Rat adrenal gland tissue using CRHR1 antibody.
Paraformaldehyde-fixed, paraffin embedded (mouse brain), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37C for 30 min, Antibody incubation with (CRHR1) Polyclonal Antibody, Unconjugated (orb182913) at 1:200 overnight at 4C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining.
Paraformaldehyde-fixed, paraffin embedded (rat brain), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37C for 30 min, Antibody incubation with (CRHR1) Polyclonal Antibody, Unconjugated (orb182913) at 1:200 overnight at 4C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining.
Sample: Lane 1: Cerebrum (Mouse) Lysate at 40 ug, Lane 2: Cerebellum (Mouse) Lysate at 40 ug, Lane 3: Testis (Mouse) Lysate at 40 ug, Lane 4: Cerebrum (Rat) Lysate at 40 ug, Lane 5: Cerebellum (Rat) Lysate at 40 ug, Primary: Anti-CRHR1 (orb182913) at 1/1000 dilution, Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution, Predicted band size: 50-55 kD, Observed band size: 48 kD.
Tissue/Cell: Rat adrenal gland, 4% Paraformaldehyde-fixed and paraffin-embedded, Antigen retrieval: citrate buffer (0.01M, pH 6.0), Boiling bathing for 15 min, Block endogenous peroxidase by 3% Hydrogen peroxide for 30 min, Blocking buffer (normal goat serum) at 37C for 20 min, Incubation: Anti-CRHR1 Polyclonal Antibody, Unconjugated (orb182913) 1:500, overnight at 4C, followed by conjugation to the secondary antibody and DAB staining.



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