GLUR2 Rabbit Polyclonal Antibody, Unconjugated
Artikelnummer:
BYT-ORB13619
- Bilder (8)
| Artikelname: | GLUR2 Rabbit Polyclonal Antibody, Unconjugated |
| Artikelnummer: | BYT-ORB13619 |
| Hersteller Artikelnummer: | orb13619 |
| Alternativnummer: | BYT-ORB13619-50,BYT-ORB13619-100,BYT-ORB13619-200 |
| Hersteller: | Biorbyt |
| Wirt: | Rabbit |
| Kategorie: | Antikörper |
| Applikation: | FC, IF, IHC-Fr, IHC-P, WB |
| Spezies Reaktivität: | Human, Mouse, Rat |
| Immunogen: | KLH conjugated synthetic peptide derived from human GluR2 (151-250/883aa) |
| Konjugation: | Unconjugated |
| Alternative Synonym: | GLUR2, GLURB, GluA2, GluR-K2, HBGR2, NEDLIB, gluR-2, gluR-B, GRIA2_HUMAN, GRIA2, AMPA-selective glutamate receptor 2, Glutamate receptor ionotropic, AMPA 2, GRIA2_MOUSE, GRIA2_RAT, |
| GLUR2 Rabbit Polyclonal Antibody |
| Klonalität: | Polyclonal |
| Konzentration: | 1mg/ml |
| Molekulargewicht: | 97 kDa |
| UniProt: | P42262 |
| Puffer: | 0.01M TBS (pH7.4) with 1% rAlbumin, 0.02% Proclin300 and 50% Glycerol. |
| Formulierung: | Liquid |
| Target-Kategorie: | GRIA2 |
| Application Verdünnung: | WB=1:500-2000, IHC-P=1:100-500, IHC-F=1:100-500, IF=1:100-500, Flow-Cyt=1µg /test |
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Blank control: RSC96 (blue), Isotype Control Antibody: Rabbit IgG (orange), Secondary Antibody: Goat anti-rabbit IgG-AF647 (white blue), Dilution: 1:100 in 1X PBS containing 0.5% BSA, Primary Antibody Dilution: 1 ugl in 100 uL 1X PBS containing 0.5% BSA (green). |
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Blank control: RSC96 (blue), Isotype Control Antibody: Rabbit IgG-AF647 (orange), Primary Antibody Dilution: 5 uL in 100 uL 1X PBS containing 0.5% BSA (green). |
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Blank control: RSC96 (blue), Isotype Control Antibody: Rabbit IgG-AF647 (orange), Primary Antibody Dilution: 5 uL in 100 uL 1X PBS containing 0.5% BSA (green). |
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Paraformaldehyde-fixed, paraffin embedded (Mouse brain), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37C for 30 min, Antibody incubation with (GLUR2) Polyclonal Antibody, Unconjugated (orb13619) at 1:500 overnight at 4C, followed by a conjugated secondary for 20 minutes and DAB staining. |
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Paraformaldehyde-fixed, paraffin embedded (Rat brain), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37C for 30 min, Antibody incubation with (GLUR2) Polyclonal Antibody, Unconjugated (orb13619) at 1:500 overnight at 4C, followed by a conjugated secondary for 20 minutes and DAB staining. |
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Sample: HepG2 Cell Lysate at 30 ug, Primary: Anti-GluR2 (orb13619) at 1/300 dilution, Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution, Predicted band size: 97 kD, Observed band size: 117 kD. |
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Tissue/Cell: mouse brain tissue, 4% Paraformaldehyde-fixed and paraffin-embedded, Antigen retrieval: citrate buffer (0.01M, pH 6.0), Boiling bathing for 15 min, Block endogenous peroxidase by 3% Hydrogen peroxide for 30 min, Blocking buffer (normal goat serum) at 37C for 20 min, Incubation: Anti-GLUR2 Polyclonal Antibody, Unconjugated (orb13619) 1:200, overnight at 4C, followed by conjugation to the secondary antibody and DAB staining. |
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Western blot:HepG2 Cell,Primary:Anti-GluR2 at 1:300 dilution. |
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