Synthetic peptide / encompassing a sequence within the center region
Konjugation:
Unconjugated
Rabbit polyclonal antibody to eNOS
Klonalität:
Polyclonal
Puffer:
100mM Tris Glycine, 20% Glycerol (pH7). 0.025% ProClin 300 was added as a preservative
Target-Kategorie:
eNOS / NOS3
Application Verdünnung:
Western Blot 1:1000-1:2000, Immunofluorescence 1:200-1:400, Immunohistochemistry (Paraffin) 1: 100-1:300, Immunohistochemistry (Frozen) 1:100-1:200
All lanes: anti-eNOS / NOS3 antibody - at 1/1000 dilution, Lysates/proteins at 40 µg per lane. This blot was produced using a 5% SDS-PAGE. The gel was run at 140V for 50 minutes before being transferred onto a Nitrocellulose membrane at 18V for 60 minutes. The membrane was then blocked an hour before being incubated with orb1294362 overnight at 4C.
All lanes: anti-eNOS / NOS3 antibody - at 1/1000 dilution, Lysates/proteins at 40 µg per lane. This blot was produced using a 5% SDS-PAGE. The gel was run at 140V for 50 minutes before being transferred onto a Nitrocellulose membrane at 18V for 60 minutes. The membrane was then blocked an hour before being incubated with orb1294362 overnight at 4C.
anti-eNOS / NOS3 antibody - at 1/1000 dilution, Lysates/proteins at 40 µg per lane. This blot was produced using a 5% SDS-PAGE. The gel was run at 140V for 50 minutes before being transferred onto a Nitrocellulose membrane at 18V for 60 minutes. The membrane was then blocked an hour before being incubated with orb1294362 overnight at 4C.
Frozen sectioned for immunohistochemical analysis. Sample: Frozen section. Red: eNOS / NOS3 antibody. Catalog: orb1294362 at 1-100.
Immunofluorescence: cells were fixed with 4% paraformaldehyde for 10 min at RT, permeabilized with 0.1% NP-40 for 10 min at RT then blocked with 5% BSA for 30 min at room temperature. Cells were stained with orb1294362 anti-eNOS/ NOS3 antibody (red) at 1:200 and 4C. DAPI (blue) was used as the nuclear counter stain.
Immunohistochemical analysis of paraffin embedded Human cancer tissue labeling eNOS with orb1294362 at 1/200.
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