Synthetic peptide / Intracellular domain corresponding to Human HER2 / ERBB2
Konjugation:
Unconjugated
Rabbit polyclonal antibody to HER2
Klonalität:
Polyclonal
Puffer:
100mM Tris Glycine, 20% Glycerol (pH7). 0.025% ProClin 300 was added as a preservative
Target-Kategorie:
HER2 / ERBB2
Application Verdünnung:
Western Blot 1:500-1:1000, Immunofluorescence 1:200-1:400, Immunohistochemistry (Paraffin) 1: 200-1:250
All lanes: Anti-HER2 / ERBB2 antibody - at 1/500 dilution, Lysates/proteins at 30 µg per lane. This blot was produced using a 5% SDS-PAGE. The gel was run at 140V for 50 minutes before being transferred onto a Nitrocellulose membrane at 18V for 60 minutes. The membrane was then blocked with 3% BSA for an hour before being incubated with orb1294317 overnight at 4C.
Immunofluorescence: cells were fixed with 4% paraformaldehyde for 10 min at RT, permeabilized with 0.1% NP-40 for 10 min at RT then blocked with 5% BSA for 30 min at room temperature. Cells were stained with orb1294317 anti-HER2 / ERBB2 antibody (red) at 1:200 and 4C. DAPI (blue) was used as the nuclear counter stain.
Immunohistochemical analysis of paraffin embedded Human Breast cancer tissue labeling HER2 / ERBB2 with orb1294317 at 1/200.
Immunohistochemical analysis of paraffin embedded Human Breast cancer tissue labeling HER2 / ERBB2 with orb1294317 at 1/200.
Immunohistochemical analysis of paraffin embedded Human Breast cancer tissue labeling HER2 / ERBB2 with orb1294317 at 1/200.
* Mehrwertsteuer und Versandkosten nicht enthalten. Irrtümer und Preisänderungen vorbehalten
