Synthetic peptide / Extracellular domain corresponding to Human CD9
Konjugation:
Unconjugated
Rabbit polyclonal antibody to CD9
Klonalität:
Polyclonal
Puffer:
100mM Tris Glycine, 20% Glycerol (pH7). 0.025% ProClin 300 was added as a preservative
Target-Kategorie:
CD9
Application Verdünnung:
Western Blot 1:500-1:800, Immunofluorescence 1:300-1:400, Immunohistochemistry (Paraffin) 1:50-1:100
CD9 antibody at 1/1000 dilution Lysates at 25 µg per lane.This blot was produced using a 12% SDS-PAGE. Nitrocellulose membrane was then blocked for an hour before being incubated with orb1294290 overnight at 4C.
CD9 antibody at 1/500 dilution Lysates at 30 µg per lane. This blot was produced using a 12% SDS-PAGE. Nitrocellulose membrane was then blocked for an hour before being incubated with orb1294290 overnight at 4C.
CD9 antibody at 1/800 dilution Lysates at 50 µg per lane. This blot was produced using a 12% SDS-PAGE. Nitrocellulose membrane was then blocked for an hour before being incubated with orb1294290 overnight at 4C.
CD9 antibody at 1/800 dilution Lysates at 50 µg per lane. This blot was produced using a 12% SDS-PAGE. Nitrocellulose membrane was then blocked for an hour before being incubated with orb1294290 overnight at 4C.
Immunofluorescence: cells were fixed with 4% PFA for 10 min at RT, permeabilized with 0.1% NP-40 for 10 min at RT then blocked with 5% BSA for 30 min at RT. Cells were stained with orb1294290 anti-CD9 antibody (red) at 1:400 and 4C. DAPI (blue) was used as the nuclear counter stain.
Immunohistochemical analysis of paraffin embedded Human cancer tissue labeling CD9 with orb1294290 at 1/100.
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