100mM Tris Glycine, 20% Glycerol (pH7). 0.025% ProClin 300 was added as a preservative
Target-Kategorie:
PPARgamma
Application Verdünnung:
Western Blot 1:500 - 1:1500, Immunofluorescence 1:300 - 1:
Anti-PPARgamma antibody at 1/1000 dilution Lysates at 40 µg per lane. This blot was produced using a 10% SDS-PAGE. Nitrocellulose membrane was then blocked an hour before being incubated with orb1294232 overnight at 4C.
Immunofluorescence: cells were fixed with 4% paraformaldehyde for 10 min at RT, permeabilized with 0.1% NP-40 for 10 min at RT then blocked with 5% BSA for 30 min at room temperature. Cells were stained with orb1294232 anti-PPARgammaantibody (red) at 1:200 and 4C. DAPI (blue) was used as the nuclear counter stain.
Immunohistochemical analysis of paraffin embedded Human breast cancer tissue labeling PPARgamma with orb1294232 at 1/100.
Immunohistochemical analysis of paraffin embedded Human breast cancer tissue labeling PPARgamma with orb1294232 at 1/100.
Immunohistochemical analysis of paraffin embedded Human cancer tissue labeling PPARgamma with orb1294232 at 1/100.
Immunohistochemical analysis of paraffin embedded Human cancer tissue labeling PPARgamma with orb1294232 at 1/100.
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