Anti-sRANK-L antibody (orb1240016) was raised against a peptide corresponding to 14 amino acids near the center of human sRANK-L. The immunogen is located within amino acids 140-190 of sRANK-L.
sRANK Ligand Antibody is supplied in PBS containing 0.02% sodium azide.
Formulierung:
Liquid
Target-Kategorie:
TNFSF11
Western Blot Validation in Human Cell Lines. Loading: 15 µg of lysates per lane. Antibodies: sRANK-L orb1240016 (1 µg/mL), 1h incubation at RT in 5% NFDM/TBST. Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10000 dilution.
Western Blot Validation in Mouse Tissues. Loading: 15 µg of lysates per lane. Antibodies: sRANK-L orb1240016 (2 µg/mL), 1h incubation at RT in 5% NFDM/TBST. Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10000 dilution.
Western Blot Validation with Recombinant Protein. Loading: 30 ng of human sRANK-L recombinant protein per lane. Antibodies: sRANK-L orb1240016 (A: 1 µg/mL, B: 2 µg/mL and C: 4 µg/mL), 1h incubation at RT in 5% NFDM/TBST. Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10000 dilution. Observed at around 27kD.
Western Blot Validation in Rat Liver Tissue. Loading: 15 µg of lysates per lane. Antibodies: sRANK-L orb1240016 (A: 0.25 µg/mL and B: 0.5 µg/mL), 1h incubation at RT in 5% NFDM/TBST. Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10000 dilution.
Immunohistochemistry Validation of sRANK-L in Human Liver Tissue. Immunohistochemical analysis of paraffin-embedded human liver tissue using anti sRANK-L antibody (orb1240016) at 5 µg/ml. Tissue was fixed with formaldehyde and blocked with 10% serum for 1 h at RT, antigen retrieval was by heat mediation with a citrate buffer (pH6). Samples were incubated with primary antibody overnight at 4C. A goat anti-rabbit IgG H&L (HRP) at 1/250 was used as secondary. Counter stained with Hematoxylin.
Immunohistochemistry and Induced Expression Validation of sRANK-L in Rat Osteogenic Compartment (Hassan et al., 2017). Protein analysis for sRANK-L by immunohistochemistry with anti-sRANK-L antibodies (orb1240016) in rat osteogenic cells. Strong immunoreactivity is shown for sRANK-L in osteogenic cells at 18hr after upper jaw molar extraction as compared to baseline.
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