Anti-KLOTHO antibody (orb1239544) was raised against a peptide corresponding to 17 amino acids near the center of human KLOTHO. The immunogen is located within amino acids 410-460 of KLOTHO.
KLOTHO Antibody is supplied in PBS containing 0.02% sodium azide.
Formulierung:
Liquid
Target-Kategorie:
KL
Independent Antibody Validation (IAV) via Protein Expression Profile in Human and Mouse Cell Lines. Loading: 15 µg of lysates per lane. Antibodies: KLOTHO orb1239544 (2 µg/mL), KLOTHO, competitor antibody (4 µg/mL) and beta-actin orb1240312 (1 µg/mL), 1h incubation at RT in 5% NFDM/TBST. Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10000 dilution.
Western Blot Validation with Recombinant Protein. Loading: 30 ng of human KLOTHO recombinant protein per lane. Antibodies: KLOTHO orb1239544 (Lane 1: 1 µg/mL, Lane 2: 2 µg/mL and Lane 3: 4 µg/mL), 1h incubation at RT in 5% NFDM/TBST. Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10000 dilution. Observed at around 72kD.
Western Blot Validation in Rat Kidney Tissue Lysate. Loading: 15 µg of lysates per lane. Antibodies: KLOTHO orb1239544 (1 µg/mL), 1h incubation at RT in 5% NFDM/TBST. Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10000 dilution.
Immunohistochemistry Validation of KLOTHO in Mouse Heart Tissue. Immunohistochemical analysis of paraffin-embedded mouse heart tissue using anti-KLOTHO antibody (orb1239544) at 2.5 µg/ml. Tissue was fixed with formaldehyde and blocked with 10% serum for 1 h at RT, antigen retrieval was by heat mediation with a citrate buffer (pH6). Samples were incubated with primary antibody overnight at 4C. A goat anti-rabbit IgG H&L (HRP) at 1/250 was used as secondary. Counter stained with Hematoxylin.
Immunofluorescence Validation of KLOTHO in Mouse Heart Tissue. Immunofluorescent analysis of 4% paraformaldehyde-fixed mouse heart tissue labeling KLOTHO with orb1239544 at 20 µg/mL, followed by goat anti-rabbit IgG secondary antibody at 1/500 dilution (red).
Immunohistochemistry Validation of KLOTHO in Rat Heart Tissue. Immunohistochemical analysis of paraffin-embedded rat heart tissue using anti-KLOTHO antibody (orb1239544) at 2.5 µg/ml. Tissue was fixed with formaldehyde and blocked with 10% serum for 1 h at RT, antigen retrieval was by heat mediation with a citrate buffer (pH6). Samples were incubated with primary antibody overnight at 4C. A goat anti-rabbit IgG H&L (HRP) at 1/250 was used as secondary. Counter stained with Hematoxylin.
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