Rabbit IgG in stabilizing components, phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. *This antibody is supplied in a stabilized formulation. Compatibility with conjugation reactions depends on the chemistry of the con
Reinheit:
Affinity-chromatography
Formulierung:
Liquid
Target-Kategorie:
Synaptosomal-associated protein 25
Application Verdünnung:
WB 1:500-2000ICC/IF 1:50-200IP 1:20
Immunofluorescent analysis using the Antibody at 1:50 dilution.
Immunofluorescent analysis using the Antibody at 1:50 dilution.
Immunofluorescent analysis using the Antibody at 1:50 dilution.
Immunofluorescent analysis using the Antibody at 1:50 dilution.
Significant genes in the spinal cord of rats with spinal cord contusion injury. (A) The fold change shows that SNCB was highest among SNAP-25, PEBP1, SNCB and AQP4 genes. (B) The expression of SNAP-25, PRBP1, SNCB and AQP4. SNAP-25 had the highest expression in both groups based on the gene array. Data are shown as the logarithmic function of 2. (C) Quantitative real time-polymerase chain reaction was used to verify the accuracy of the gene array. The levels of SNAP-25, PRBP1, SNCB and AQP4 were significantly decreased in the SCI group when compared with the sham group, and SNAP-25 was most highly expressed in the two groups. Thus, SNAP-25 was selected for further research in this study. Data are relative to beta-actin. Data are presented as the mean SEM and were analyzed using Students t -test. *** P < 0.001. SCI: Spinal cord injury, SNAP-25: synaptosomal-associated protein 25 kDa, PEBP1: phosphatidylethanolamine binding protein 1, SNCB: beta-synuclein, AQP4: aquaporin 4.Index in PubMed under a CC BY license. PMID: 28761431
SNAP-25 expression in rats with spinal cord injury. (A) SNAP-25 expression in spinal cord tissue of rats detected by quantitative real time-polymerase chain reaction. Data are relative to beta-actin. Data are presented as the mean SEM and were analyzed using one-way analysis of variance with Dunnett T3. (B) Western blot assay was used to detect SNAP-25 protein expression in the spinal cord. Data represent the optical density ratio to beta-actin. Data are presented as the mean SEM and were analyzed using one-way analysis of variance with the least significant difference. * P < 0.05, vs . sham group. SNAP-25: Synaptosomal-associated protein 25 kDa, d: day(s).Index in PubMed under a CC BY license. PMID: 28761431
Distribution of SNAP-25 in the posterior horn of spinal cord. Immunohistochemistry was performed to investigate the location of SNAP-25 in the spinal cord of rat. (A) The region of SNAP-25-immunoreactivity located in the posterior horn of spinal cord. The squares correspond to the location of images in B-G. (B-G) A large amount of immunoreactivity was found on axons of neurons and glial cells in the dorsal horn of the spinal cord in both the sham and spinal cord injury groups. Scale bar: 100 µm. Black arrows indicate SNAP-25 immunoreactivity. (H) Quantitative results of the density of SNAP-25 immunoreactivity. Data are presented as the mean SEM and were analyzed using one-way analysis of variance with the least significant difference. * P < 0.05, vs
Western blot analysis of SNAP25 using anti-SNAP25 antibody (M01625). Electrophoresis was performed on a 12% SDS-PAGE gel at 80V (Stacking gel) / 120V (Resolving gel) for 2 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: mouse brain tissue lysates,Lane 2: mouse Neuro-2a whole cell lysates.After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-SNAP25 antigen affinity purified monoclonal antibody (M01625) at 1:500 overnight at 4C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an ECL Plus Western Blotting Substrate (Catalog AR1196-200) with Tanon 5200 system. A specific band was detected for SNAP25 at approximately 25 kDa. The expected band size for SNAP25 is at 23 kDa.
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