Boster Bio Anti-Hsc70 HSPA8 Rabbit Monoclonal Antibody catalog M01024. Tested in WB, IHC, ICC/IF, IP, Flow Cytometry applications. This antibody reacts with Human, Mouse, Rat.
Rabbit IgG in stabilizing components, phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. *This antibody is supplied in a stabilized formulation. Compatibility with conjugation reactions depends on the chemistry of the con
Western blot analysis of HSC70/HSPA8 using anti-HSC70/HSPA8 antibody (M01024). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human Hela whole cell lysates, Lane 2: human 293T whole cell lysates, Lane 3: human Caco-2 whole cell lysates, Lane 4: human A549 whole cell lysates, Lane 5: rat heart tissue lysates, Lane 6: rat liver tissue lysates, Lane 7: mouse heart tissue lysates, Lane 8: mouse liver tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-HSC70/HSPA8 antigen affinity purified monoclonal antibody (M01024) at 1:500 overnight at 4C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:500 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog EK1002) with Tanon 5200 system. A specific band was detected for HSC70/HSPA8 at approximately 71 kDa. The expected band size for HSC70/HSPA8 is at 71 kDa.
Immunofluorescent analysis using the Antibody at 1:50 dilution.
Immunofluorescent analysis of MCF7 cells, using Hsc70 Antibody .
Immunohistochemical analysis of paraffin-embedded human breast cancer, using Hsc70 Antibody.
Immunohistochemical analysis of paraffin-embedded Human breast cancer, using the Antibody at 1:500 dilution.
Immunohistochemical analysis of paraffin-embedded Mouse lung, using the Antibody at 1:200 dilution.
Immunohistochemical analysis of paraffin-embedded Rat liver, using the Antibody at 1:200 dilution.
Immunohistochemical analysis of paraffin-embedded Rat ovary, using the Antibody at 1:200 dilution.
Immunohistochemical analysis of paraffin-embedded Human liver cancer, using the Antibody at 1:500 dilution.
* Mehrwertsteuer und Versandkosten nicht enthalten. Irrtümer und Preisänderungen vorbehalten
