E.coli-derived human Poliovirus Receptor/PVR recombinant protein (Position: D28-E331).
Alternative Synonym:
CD155, HVED, Necl 5, NECL5, Nectin like protein 5, Poliovirus receptor, PVR, PVS, TAGE4
Boster Bio Anti-Poliovirus Receptor/PVR Antibody Picoband (monoclonal, 5I13D1) catalog M00664-2. Tested in Flow Cytometry, IHC, WB applications. This antibody reacts with Human, Mouse. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
Klonalität:
Monoclonal
Konzentration:
Adding 0.2 ml of distilled water will yield a concentration of 500 µg/ml.
Each vial contains 4 mg Trehalose, 0.9 mg NaCl and 0.2 mg Na2HPO4.
Reinheit:
Immunogen affinity purified.
Formulierung:
Lyophilized
Target-Kategorie:
Poliovirus receptor
Application Verdünnung:
Western blot, 0.25-0.5 µg/ml, Human Immunohistochemistry(Paraffin-embedded Section), 2-5 µg/ml, Human, Mouse Flow Cytometry (Fixed), 1-3 µg/1x106 cells, Human
IHC analysis of Poliovirus Receptor/PVR using anti-Poliovirus Receptor/PVR antibody (M00664-2). Poliovirus Receptor/PVR was detected in a paraffin-embedded section of human colorectal adenocarcinoma tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 µg/ml mouse anti-Poliovirus Receptor/PVR Antibody (M00664-2) overnight at 4C. Peroxidase Conjugated Goat Anti-mouse IgG was used as secondary antibody and incubated for 30 minutes at 37C. The tissue section was developed using HRP Conjugated Mouse IgG Super Vision Assay Kit (Catalog SV0001) with DAB as the chromogen.
IHC analysis of Poliovirus Receptor/PVR using anti-Poliovirus Receptor/PVR antibody (M00664-2). Poliovirus Receptor/PVR was detected in a paraffin-embedded section of human endometrial cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 µg/ml mouse anti-Poliovirus Receptor/PVR Antibody (M00664-2) overnight at 4C. Peroxidase Conjugated Goat Anti-mouse IgG was used as secondary antibody and incubated for 30 minutes at 37C. The tissue section was developed using HRP Conjugated Mouse IgG Super Vision Assay Kit (Catalog SV0001) with DAB as the chromogen.
IHC analysis of Poliovirus Receptor/PVR using anti-Poliovirus Receptor/PVR antibody (M00664-2). Poliovirus Receptor/PVR was detected in a paraffin-embedded section of human laryngeal squamous cell carcinoma tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 µg/ml mouse anti-Poliovirus Receptor/PVR Antibody (M00664-2) overnight at 4C. Peroxidase Conjugated Goat Anti-mouse IgG was used as secondary antibody and incubated for 30 minutes at 37C. The tissue section was developed using HRP Conjugated Mouse IgG Super Vision Assay Kit (Catalog SV0001) with DAB as the chromogen.
IHC analysis of Poliovirus Receptor/PVR using anti-Poliovirus Receptor/PVR antibody (M00664-2). Poliovirus Receptor/PVR was detected in a paraffin-embedded section of human liver cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 µg/ml mouse anti-Poliovirus Receptor/PVR Antibody (M00664-2) overnight at 4C. Peroxidase Conjugated Goat Anti-mouse IgG was used as sec
Western blot analysis of Poliovirus Receptor/PVR using anti-Poliovirus Receptor/PVR antibody (M00664-2). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human A431 whole cell lysates, Lane 2: human A549 whole cell lysates, Lane 3: human HT1080 whole cell lysates, Lane 4: human Daudi whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with mouse anti-Poliovirus Receptor/PVR antigen affinity purified monoclonal antibody (Catalog M00664-2) at 0.5 µg/mL overnight at 4C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-mouse IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog EK1001) with Tanon 5200 system. A specific band was detected for Poliovirus Receptor/PVR at approximately 70-80 kDa. The expected band size for Poliovirus Receptor/PVR is at 45 kDa.
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