E.coli-derived human TIM10/TIMM10 recombinant protein (Position: M1-A90). Human TIM10/TIMM10 shares 100% amino acid (aa) sequence identity with both mouse and rat TIM10/TIMM10.
Alternative Synonym:
TIMM10, TIM10A, TIMM10A
Boster Bio Anti-TIM10/TIMM10 Antibody Picoband catalog A11593-1. Tested in WB, IHC, IF, ICC/IF, Flow Cytometry, ELISA applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
Klonalität:
Polyclonal
Konzentration:
Adding 0.2 ml of distilled water will yield a concentration of 500 µg/ml.
Western blot, 0.25-0.5 µg/ml, Human, Mouse, Rat Immunohistochemistry (Paraffin-embedded Section), 2-5 µg/ml, Human Immunofluorescence, 5 µg/ml, Rat Immunocytochemistry/Immunofluorescence, 5 µg/ml, Human Flow Cytometry (Fixed), 1-3 µg/1x106 cells, Human, M
IF analysis of TIM10/TIMM10 using anti-TIM10/TIMM10 antibody (A11593-1). TIM10/TIMM10 was detected in a paraffin-embedded section of rat brain tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 5 µg/mL rabbit anti-TIM10/TIMM10 Antibody (A11593-1) overnight at 4C. Cy3 Conjugated Goat Anti-Rabbit IgG (BA1032) was used as secondary antibody at 1:500 dilution and incubated for 30 minutes at 37C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and
IHC analysis of TIM10/TIMM10 using anti-TIM10/TIMM10 antibody (A11593-1). TIM10/TIMM10 was detected in a paraffin-embedded section of human liver cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 µg/ml rabbit anti-TIM10/TIMM10 Antibody (A11593-1) overnight at 4C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog SV0002) with DAB as the chromogen.
IHC analysis of TIM10/TIMM10 using anti-TIM10/TIMM10 antibody (A11593-1). TIM10/TIMM10 was detected in a paraffin-embedded section of human liver tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 µg/ml rabbit anti-TIM10/TIMM10 Antibody (A11593-1) overnight at 4C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog SV0002) with DAB as the chromogen.
IHC analysis of TIM10/TIMM10 using anti-TIM10/TIMM10 antibody (A11593-1). TIM10/TIMM10 was detected in a paraffin-embedded section of human lung cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 µg/ml rabbit anti-TIM10/TIMM10 Antibody (A11593-1) overnight at 4C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog SV0002) with DAB as the chromogen.
Western blot analysis of TIM10/TIMM10 using anti-TIM10/TIMM10 antibody (A11593-1). Electrophoresis was performed on a 12% SDS-PAGE gel at 80V (Stacking gel) / 120V (Resolving gel) for 2 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human A549 whole cell lysates,Lane 2: human PC-3 whole cell lysates,Lane 3: human REH whole cell lysates,Lane 4: human U2OS whole cell lysates,Lane 5: rat heart tissue lysates,Lane 6: rat liver tissue lysates,Lane 7: mouse heart tissue lysates,Lane 8: mouse liver tissue lysates.After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-TIM10/TIMM10 antigen affinity purified polyclonal antibody (A11593-1) at 0.5 µg/mL overnight at 4C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an ECL Plus Western Blotting Substrate (Catalog AR1196-200) with Tanon 5200 system. A specific band was detected for TIM10/TIMM10 at approximately 10 kDa. The expected band size for TIM10/TIMM10 is at 10 kDa.
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