E.coli-derived human RPL29 recombinant protein (Position: Q27-A153).
Alternative Synonym:
60S ribosomal protein L29, HIP, HUMRPL29, ribosomal protein L29, RPL29
Boster Bio Anti-RPL29 Antibody Picoband catalog A05949-1. Tested in ELISA, Flow Cytometry, IF, IHC, ICC, WB applications. This antibody reacts with Human, Mouse, Rat, Monkey. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
Klonalität:
Polyclonal
Konzentration:
Adding 0.2 ml of distilled water will yield a concentration of 500 µg/ml.
Western blot, 0.1-0.25 µg/ml, Human, Mouse, Rat, Monkey Immunohistochemistry(Paraffin-embedded Section), 2-5 µg/ml, Human, Mouse, Rat Immunocytochemistry/Immunofluorescence, 5 µg/ml, Human Flow Cytometry (Fixed), 1-3 µg/1x106 cells, Human ELISA, 0.1-0.5 µ
IHC analysis of RPL29 using anti-RPL29 antibody (A05949-1). RPL29 was detected in a paraffin-embedded section of mouse brain tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 µg/ml rabbit anti-RPL29 Antibody (A05949-1) overnight at 4C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog SA1022) with DAB as the chromogen.
Western blot analysis of RPL29 using anti-RPL29 antibody (A05949-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: mouse NIH/3T3 whole cell lysates, Lane 2: monkey COS-7 whole cell lysates, Lane 3: human Hela whole cell lysates, Lane 4: human HepG2 whole cell lysates, Lane 5: rat pancreas tissue lysates, Lane 6: rat C6 whole cell lysates, Lane 7: mouse pancreas tissue lysates, Lane 8: mouse RAW264.7 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-RPL29 antigen affinity purified polyclonal antibody (Catalog A05949-1) at 0.25 µg/mL overnight at 4C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog EK1002) with Tanon 5200 system. A specific band was detected for RPL29 at approximately 20 kDa. The expected band size for RPL29 is at 20 kDa.
IHC analysis of RPL29 using anti-RPL29 antibody (A05949-1). RPL29 was detected in a paraffin-embedded section of rat brain tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 µg/ml rabbit anti-RPL29 Antibody (A05949-1) overnight at 4C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog SA1022) with DAB as the chromogen.
Flow Cytometry analysis of Hela cells using anti-RPL29 antibody (A05949-1). Overlay histogram showing Hela cells stained with A05949-1 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-RPL29 Antibody (A05949-1, 1 µg/1x106 cells) for 30 min at 20C. DyLight488 conjugated goat anti-rabbit IgG (BA1127, 5-10 µg/1x106 cells) was used as secondary antibody for 30 minutes at 20C. Isotype control antibody (Green line) was rabbit IgG (1 µg/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondar
IF analysis of RPL29 using anti-RPL29 antibody (A05949-1). RPL29 was detected in an immunocytochemical section of MCF-7 cells. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent (AR0022) for 15 mins. The cells were blocked with 10% goat serum. And then incubated with 5 µg/mL rabbit anti-RPL29 Antibody (A05949-1) overnight at 4C. DyLight594 Conjugated Goat Anti-Rabbit IgG (BA1142) was used as secondary antibody at 1:100 dilution and incubated for 30 minutes at 37C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.
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