Monoclonal Antibody to RIG-I (Clone: ABM40B5)
Artikelnummer:
ABI-10-4102
- Bilder (3)
| Artikelname: | Monoclonal Antibody to RIG-I (Clone: ABM40B5) |
| Artikelnummer: | ABI-10-4102 |
| Hersteller Artikelnummer: | 10-4102 |
| Alternativnummer: | ABI-10-4102-100UG |
| Hersteller: | Abeomics |
| Wirt: | Mouse |
| Kategorie: | Antikörper |
| Applikation: | FACS, IHC, WB |
| Spezies Reaktivität: | Human |
| Immunogen: | A partial length recombinant human RIG-I protein (amino acids 1-220) was used as the immunogen for this antibody. |
| Alternative Synonym: | DDX58 |
| RIG-I or Probable ATP-dependent RNA helicase DDX58 or simply DDX58 is a RNA helicase enzyme with two isoforms (106 and 101 kDa). RIG-I belongs to the DEAD/H box family and is involved in several cellular responses including RNA binding and alteration of RNA secondary structure. RIG-1 acts as sensor of viral nucleic acids thus activating a cascade of antiviral responses including the induction of type I interferons and pro-inflammatory cytokines. Generally it remains as a monomer but upon activation by viral dsRNA it homomultimerizes and interacts with mitochondria antiviral signaling protein (MAVS/IPS1) which activating a cascade of reactions to produce specific interferon. RIG-1 is mostly expressed in vascular smooth cells. |
| Application Verdünnung: | Western blot analysis: 2-4 µg/ml, Immunohistochemical analysis: 5 µg/ml, Flowcytometric analysis: 0.5-1µg/10 6 cells |
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Fig-1: Western blot analysis of RIG-I. Anti- RIG-I antibody (Clone: ABM40B5) was used at 2 µg/ml on K562 lysates. |
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Fig-2 : Immunohistochemical analysis of RIG-I in human prostate tissue using RIG-I antibody (Clone: ABM40B5) at 5 µg/ml. |
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Figure-3: Intracellular flow cytometric analysis of RIG-I in K562 Cell line using 0.5 µg/10 6 cells of Anti-RIGI antibody (ABM40B5). Green represent isotype control and red represent Anti-RIG I antibody (10-4102 Abeomics). Goat anti-mouse PE conjugate was used as secondary. |
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