Monoclonal antibody to Cytochrome C (Clone: ABM30B3)
Artikelnummer:
ABI-10-1050-25
Hersteller Artikelnummer:
10-1050-25
Alternativnummer:
ABI-10-1050-25-25UG
Hersteller:
Abeomics
Wirt:
Mouse
Kategorie:
Antikörper
Applikation:
FACS, WB
Spezies Reaktivität:
Human, Mouse, Rat
Immunogen:
Full length recombinant Cytochrome C protein was used as the immunogen for this antibody.
Alternative Synonym:
CYCS||CYC
Cytochrome C is an inner mitochondrial membrane hemeprotein encoded by CYCS gene. Cytochrome C has two major roles within the cell, firstly, it acts as an electron carrier between Complex III (Cytochrome bc1 complex) and Complex IV (Cytochrome C oxidase). The second important function of Cytochrome C is in apoptosis. In response to extrinsic or intrinsic signals and upon suppression of anti-apoptotic molecules, several pro-apoptotic molecules are activated that disturb the mitochondrial membrane permeability and causes the release of Cytochrome C. Cytochrome C then binds to apoptotic protease activating factor-1 (Apaf-1). This complex interacts with pro-caspase 9 and initiates the caspase cascade resulting in apoptosis. Human Cytochrome C has a molecular mass of 11 kDa. Defect in the CYCS gene is associated with autosomal dominant diseases like thrombocytopenia type 4 (THC4). High levels of Cytochrome C are found in blood leukocytes, kidney, liver, heart and brain.
Western blot analysis:2-4 µg/ml, FACS Analysis: 0.5-1 µg/10 6 cells
Figure:1- Western blot analysis of Cytochrome C. Anti-Cytochrome C antibody (Clone: ABM30B3) was tested at 2 µg/ml on h Kidney lysate.
Figure:2- Intracellular Flow analysis of Cytochrome C antibody in A431 cells using 0.5 µg/ 10 6 cells of anti-Cytochrome C antibody (ABM30B3). Green represents isotype control,red represents anti-Cytochrome C antibody. Goat anti-mouse PE conjugate was used as secondary antibody.
Figure:3- Western blot analysis of Cytochrome C. Anti-Cytochrome C antibody (Clone: ABM30B3) was tested at 2 µg/ml on (1) m Kidney, (2) r Kidney and (3) 3T3 lysates.
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