Monoclonal Antibody to ATF6 (Clone: ABM1A40)
Artikelnummer:
ABI-10-1018
- Bilder (3)
| Artikelname: | Monoclonal Antibody to ATF6 (Clone: ABM1A40) |
| Artikelnummer: | ABI-10-1018 |
| Hersteller Artikelnummer: | 10-1018 |
| Alternativnummer: | ABI-10-1018-100UG |
| Hersteller: | Abeomics |
| Wirt: | Mouse |
| Kategorie: | Antikörper |
| Applikation: | FACS, WB |
| Spezies Reaktivität: | Human |
| Immunogen: | A partial length recombinant ATF6 protein (amino acids 1-400) was used as the immunogen for this antibody. |
| Alternative Synonym: | ATF6 |
| ATF6, also named as Cyclic AMP-dependent transcription factor ATF-6 alpha is a member of bZIP family, ATF subfamily. ATF6 forms homodimer and heterodimer with ATF6-beta. ATF6 acts by activating specific unfolded protein response target genes during endoplasmic reticulum stress. ATF6 is a ubiquitously expressed transcription factor located at endoplasmic reticulum (ER) membrane. During ER stress an approximately 50 kDa fragment containing the N-terminal cytoplasmic domain is cleaved by proteolysis and is translocated into the nucleus. N-glycosylation may serve as a sensor for ER homeostasis. For the binding of ATF6 to ERSE, ESRE should first bind to another transcription factor called Nuclear Transcription Factor Y (NF-Y). ATF6 could also be involved in activation of transcription by interacting with serum response factor. ATF6 is a 74 kDa protein and is highly expressed in kidney, liver, colon and mammary gland. |
| Application Verdünnung: | Western blot analysis: 2-4 µg/ml, FACS: 0.5-1 µg/10 6 cells |
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Fig-1: Expression analysis of ATF6. Anti- ATF6 antibody (Clone ABM1A40) was used at 2 µg/ml on K562 lysate. |
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Fig-2: Intracellular staining of full length ATF6 plasmid and blank plasmid transfected HEK293 cells. 0.5 ug of anti-ATF6 antibody was used. Green represents blank plasmid, Red represents ATF6 expression plasmid. Goat anti-mouse PE was used as secondary antibody. |
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Fig-3: Expression analysis of ATF6. Anti- ATF6 antibody (Clone ABM1A40) was used at 2 µg/ml on (1) Blank plasmid transfected HEK293 cells, (2) Partial length ATF6 plasmid transfected HEK293 cells and (3) Fulll length ATF6 plasmid transfected HEK293 cells. |
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