Phospho-Histone H3-S10 Rabbit mAb, Unconjugated

Artikelname:	Phospho-Histone H3-S10 Rabbit mAb, Unconjugated
Artikelnummer:	ABB-AP1586
Hersteller Artikelnummer:	AP1586
Alternativnummer:	ABB-AP1586-500UL,ABB-AP1586-100UL,ABB-AP1586-1000UL,ABB-AP1586-20UL
Hersteller:	ABclonal
Wirt:	Rabbit
Kategorie:	Antikörper
Applikation:	DOT, ELISA, IHC-P, IP, WB
Spezies Reaktivität:	Human
Immunogen:	Synthetic peptide. This information is considered to be commercially sensitive.
Konjugation:	Unconjugated
Alternative Synonym:	H3/A, H3C2, H3C3, H3C4, H3C6, H3C7, H3C8, H3FA, H3C10, H3C11, H3C12, HIST1H3A, H3t, H3.4, H3/g, H3FT, H3C16, HIST3H3

Histones are basic nuclear proteins that are responsible for the nucleosome structure of the chromosomal fiber in eukaryotes. Nucleosomes consist of approximately 146 bp of DNA wrapped around a histone octamer composed of pairs of each of the four core histones (H2A, H2B, H3, and H4). The chromatin fiber is further compacted through the interaction of a linker histone, H1, with the DNA between the nucleosomes to form higher order chromatin structures. This gene is intronless and encodes a replication-dependent histone that is a member of the histone H3 family. Transcripts from this gene lack polyA tails, instead, they contain a palindromic termination element. This gene is located separately from the other H3 genes that are in the histone gene cluster on chromosome 6p22-p21.3.

Molekulargewicht:	15 kDa
NCBI:	8290
UniProt:	Q16695
Reinheit:	Affinity purification
Sequenz:	ARKST
Target-Kategorie:	H3C1/H3-4

Application Verdünnung:	WB,1:5000 - 1:20000\|IHC-P,1:500 - 1:2000\|IP,0.5µg-4µg antibody for 200µg-400µg extracts of whole cells\|DB,1:2000 - 1:5000\|ELISA,Recommended starting concentration is 1 µg/mL. Please optimize the concentration based on your specific assay requirements. For
Anwendungsbeschreibung:	Cross-Reactivity: Human,Mouse,Rat,Other (Wide Range Predicted). ResearchArea: Epigenetics Nuclear Signaling,Protein phosphorylation,Signal Transduction,MAPK-Erk Signaling Pathway. Shipping: Ice Bag

	Western blot analysis of lysates from HeLa cells using Phospho-Histone H3-S10 Rabbit mAb (AP1586) at 1:6000 dilutionincubated overnight at 4°C. HeLa cells were treated with Nocodazole (100 ng/mL) at 37°C for 17 hours. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution. Lysates/proteins: 30 µg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 20 s.
	Western blot analysis of lysates from NIH/3T3 cells using Phospho-Histone H3-S10 Rabbit mAb (AP1586) at 1:6000 dilutionincubated overnight at 4°C. NIH/3T3 cells were treated with Nocodazole (100 ng/mL) at 37°C for 17 hours. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution. Lysates/proteins: 30 µg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 30 s.
	Western blot analysis of lysates from C6 cells using Phospho-Histone H3-S10 Rabbit mAb (AP1586) at 1:6000 dilutionincubated overnight at 4°C. C6 cells were treated with Nocodazole (100 ng/mL) at 37°C for 17 hours, or treated with Nocodazole (100 ng/mL) at 37°C for 17 hours and lambda-PP (2 U/µL) at 37°C for 1 hour. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution. Lysates/proteins: 30 µg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 10 s.
	Dot-blot analysis of all sorts of peptides using Phospho-Histone H3-S10 Rabbit mAb (AP1586) at 1:5000 dilution incubated overnight at 4°C.
	Immunohistochemistry analysis of paraffin-embedded Human cervix tissue, untreated (left) and lambda phosphatase-treated (right), using Phospho-Histone H3-S10 Rabbit mAb (AP1586) at a dilution of 1:500 (40x lens). High pressure antigen retrieval performed with 0.01M Tris-EDTA Buffer (pH 9.0) prior to IHC staining.
	Immunohistochemistry analysis of paraffin-embedded Human colon carcinoma tissue, untreated (left) and lambda phosphatase-treated (right), using Phospho-Histone H3-S10 Rabbit mAb (AP1586) at a dilution of 1:500 (40x lens). High pressure antigen retrieval performed with 0.01M Tris-EDTA Buffer (pH 9.0) prior to IHC staining.
	Immunohistochemistry analysis of paraffin-embedded Mouse testis tissue, untreated (left) and lambda phosphatase-treated (right), using Phospho-Histone H3-S10 Rabbit mAb (AP1586) at a dilution of 1:500 (40x lens). High pressure antigen retrieval performed with 0.01M Tris-EDTA Buffer (pH 9.0) prior to IHC staining.
	Immunohistochemistry analysis of paraffin-embedded Rat testis tissue, untreated (left) and lambda phosphatase-treated (right), using Phospho-Histone H3-S10 Rabbit mAb (AP1586) at a dilution of 1:500 (40x lens). High pressure antigen retrieval performed with 0.01M Tris-EDTA Buffer (pH 9.0) prior to IHC staining.
	Immunoprecipitation of Phospho-Histone H3-S10 from 300 µg extracts of HeLa cells was performed using 2 µg of Phospho-Histone H3-S10 Rabbit mAb (AP1586). Rabbit Control IgG (AC005) was used to precipitate the Control IgG sample. IP samples were eluted with 1X Laemmli Buffer. The Input lane represents 10% of the total input. Western blot analysis of immunoprecipitates was conducted using Phospho-Histone H3-S10 Rabbit mAb (AP1586) at a dilution of 1:10000.