DNA Ligase I Rabbit mAb, Unconjugated, Monoclonal

Artikelname:	DNA Ligase I Rabbit mAb, Unconjugated, Monoclonal
Artikelnummer:	ABB-A9301
Hersteller Artikelnummer:	A9301
Alternativnummer:	ABB-A9301-100UL,ABB-A9301-20UL
Hersteller:	ABclonal
Wirt:	Rabbit
Kategorie:	Antikörper
Applikation:	ELISA, IF, IHC-P, WB
Spezies Reaktivität:	Human
Immunogen:	Recombinant protein (or fragment).This information is considered to be commercially sensitive.
Konjugation:	Unconjugated
Alternative Synonym:	LIGI, IMD96, hLig1, DNA Ligase I

This gene encodes a member of the ATP-dependent DNA ligase protein family. The encoded protein functions in DNA replication, recombination, and the base excision repair process. Mutations in this gene that lead to DNA ligase I deficiency result in immunodeficiency and increased sensitivity to DNA-damaging agents. Disruption of this gene may also be associated with a variety of cancers. Alternative splicing results in multiple transcript variants.

Klonalität:	Monoclonal
Molekulargewicht:	102kDa
NCBI:	3978
UniProt:	P18858
Reinheit:	Affinity purification
Target-Kategorie:	LIG1

Application Verdünnung:

WB,1:500 - 1:1000|IHC-P,1:50 - 1:200|IF/ICC,1:50 - 1:200|ELISA,Recommended starting concentration is 1 µg/mL. Please optimize the concentration based on your specific assay requirements.

Anwendungsbeschreibung:

Cross-Reactivity: Human,Mouse,Rat. ResearchArea: chromatin DNA binding,metal ion binding,methyltransferase activity,RNA polymerase II transcription regulatory region sequence-specific DNA binding,cell fate specification,fibroblast growth factor receptor signaling pathway,germ-line stem cell population maintenance,histone H3-R26 methylation,homeostasis of number of cells within a tissue,inactivation of paternal X chromosome,inner cell mass cell fate commitment,negative regulation of fibroblast growth factor receptor signaling pathway,negative regulation of transcription by RNA polymerase II,ositive regulation of flagellated sperm motility,positive regulation of stem cell population maintenance,regulation of DNA methylation. Shipping: Ice Bag

	Western blot analysis of lysates from Jurkat cells, using DNA Ligase I Rabbit mAb (A9301) at 1:1000 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution. Lysates/proteins: 25µg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 30s.
	Immunohistochemistry analysis of paraffin-embeddedRat lung tissue usingDNA Ligase I Rabbit mAb(A9301) at a dilution of 1:200 (40x lens).High pressure antigen retrieval was performed with 0.01 M citrate buffer (pH 6.0) prior to IHC staining.
	Immunohistochemistry analysis of paraffin-embeddedHuman spleen tissue usingDNA Ligase I Rabbit mAb(A9301) at a dilution of 1:200 (40x lens).High pressure antigen retrieval was performed with 0.01 M citrate buffer (pH 6.0) prior to IHC staining.
	Immunohistochemistry analysis of paraffin-embeddedRat brain tissue usingDNA Ligase I Rabbit mAb(A9301) at a dilution of 1:200 (40x lens).High pressure antigen retrieval was performed with 0.01 M citrate buffer (pH 6.0) prior to IHC staining.
	Immunohistochemistry analysis of paraffin-embeddedHuman thyroid cancer tissue usingDNA Ligase I Rabbit mAb(A9301) at a dilution of 1:200 (40x lens).High pressure antigen retrieval was performed with 0.01 M citrate buffer (pH 6.0) prior to IHC staining.
	Immunohistochemistry analysis of paraffin-embeddedMouse brain tissue usingDNA Ligase I Rabbit mAb(A9301) at a dilution of 1:200 (40x lens).High pressure antigen retrieval was performed with 0.01 M citrate buffer (pH 6.0) prior to IHC staining.
	Immunohistochemistry analysis of paraffin-embeddedHuman colon carcinoma tissue usingDNA Ligase I Rabbit mAb(A9301) at a dilution of 1:200 (40x lens).High pressure antigen retrieval was performed with 0.01 M citrate buffer (pH 6.0) prior to IHC staining.
	Immunofluorescence analysis of C6 cells using DNA Ligase I Rabbit mAb (A9301) at dilution of 1:100 (40x lens). Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.
	Immunofluorescence analysis of U-2 OS cells using DNA Ligase I Rabbit mAb (A9301) at dilution of 1:100 (40x lens). Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.