CD99 Rabbit mAb, Unconjugated, Monoclonal

Artikelname:	CD99 Rabbit mAb, Unconjugated, Monoclonal
Artikelnummer:	ABB-A25856
Hersteller Artikelnummer:	A25856
Alternativnummer:	ABB-A25856-20UL,ABB-A25856-100UL
Hersteller:	ABclonal
Wirt:	Rabbit
Kategorie:	Antikörper
Applikation:	ELISA, FC, IF, IHC-P, WB
Spezies Reaktivität:	Human
Immunogen:	Synthetic peptide. This information is considered to be commercially sensitive.
Konjugation:	Unconjugated
Alternative Synonym:	MIC2, HBA71, MIC2X, MIC2Y, MSK5X

The protein encoded by this gene is a cell surface glycoprotein involved in leukocyte migration, T-cell adhesion, ganglioside GM1 and transmembrane protein transport, and T-cell death by a caspase-independent pathway. In addition, the encoded protein may have the ability to rearrange the actin cytoskeleton and may also act as an oncosuppressor in osteosarcoma. This gene is found in the pseudoautosomal region of chromosomes X and Y and escapes X-chromosome inactivation. There is a related pseudogene located immediately adjacent to this locus.

Klonalität:	Monoclonal
Molekulargewicht:	16-19kDa
NCBI:	4267
UniProt:	P14209
Reinheit:	Affinity purification
Target-Kategorie:	CD99

Application Verdünnung:	WB,1:2000 - 1:8000\|IHC-P,1:20000 - 1:80000\|IF/ICC,1:50 - 1:2000\|FC,1:500 - 1:1000\|ELISA,Recommended starting concentration is 1 µg/mL. Please optimize the concentration based on your specific assay requirements.
Anwendungsbeschreibung:	Cross-Reactivity: Human. ResearchArea: Cancer,Invasion and Metastasis,Signal Transduction,Cell Biology Developmental Biology,Cell Adhesion,Cytoskeleton,Immunology Inflammation,CDs. Shipping: Ice Bag

	Western blot analysis of lysates from Hep G2 cells using CD99 Rabbit mAb (A25856) at 1:2000 dilution incubated overnight at 4°C. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution. Lysates/proteins: 25 µg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 30s.
	Immunohistochemistry analysis of paraffin-embedded Human ewing sarcoma tissue using CD99 Rabbit mAb (A25856) at a dilution of 1:30000 (40x lens). High pressure antigen retrieval performed with 0.01M Tris-EDTA Buffer (pH 9.0) prior to IHC staining.
	Immunohistochemistry analysis of paraffin-embedded Human liver tissue using CD99 Rabbit mAb (A25856) at a dilution of 1:30000 (40x lens). High pressure antigen retrieval performed with 0.01M Tris-EDTA Buffer (pH 9.0) prior to IHC staining.
	Confocal imaging of Hep G2 cells using CD99 Rabbit mAb (A25856, dilution 1:200) followed by a further incubation with Cy3 Goat Anti-Rabbit IgG (H+L) (AS007, dilution 1:500) (Red). The cells were counterstained with alpha-Tubulin Mouse mAb (AC012, dilution 1:400) followed by incubation with ABflo 488-conjugated Goat Anti-Mouse IgG (H+L) Ab (AS076, dilution 1:500) (Green). DAPI was used for nuclear staining (Blue). Objective: 100x.
	Confocal imaging of MOLT-4 cells using CD99 Rabbit mAb (A25856, dilution 1:200) followed by a further incubation with Cy3 Goat Anti-Rabbit IgG (H+L) (AS007, dilution 1:500) (Red). DAPI was used for nuclear staining (Blue). Objective: 100x.
	Confocal imaging of Jurkat cells using CD99 Rabbit mAb (A25856, dilution 1:200) followed by a further incubation with Cy3 Goat Anti-Rabbit IgG (H+L) (AS007, dilution 1:500) (Red). DAPI was used for nuclear staining (Blue). Objective: 100x.
	Flow cytometry: 1X10 6 U266 cells (negative control,left)and Jurkat cells (right) cells were surface-stained with CD99 Rabbit mAb (A25856,2 µg/mL,orange line) or Rabbit IgG isotype control (AC042,2 µg/mL,blue line), followed by PE conjugated Donkey anti-rabbit pAb staining. Non-fluorescently stained cells were used as blank control (red line).
	Flow cytometry: 1X10 6 Jurkat cells were surface-stained with Rabbit IgG isotype control (AC042,2 µg/mL,left) or CD99 Rabbit mAb (A25856,2 µg/mL,right), followed by PE conjugated Donkey anti-rabbit pAb staining.