ICAM3/CD50 Rabbit mAb, Unconjugated, Monoclonal

Artikelname:	ICAM3/CD50 Rabbit mAb, Unconjugated, Monoclonal
Artikelnummer:	ABB-A25722
Hersteller Artikelnummer:	A25722
Alternativnummer:	ABB-A25722-100UL,ABB-A25722-20UL
Hersteller:	ABclonal
Wirt:	Rabbit
Kategorie:	Antikörper
Applikation:	ELISA, FC, IF, IHC-P, WB
Spezies Reaktivität:	Human
Immunogen:	Recombinant protein (or fragment).This information is considered to be commercially sensitive.
Konjugation:	Unconjugated
Alternative Synonym:	CD50, CDW50, ICAM-R

The protein encoded by this gene is a member of the intercellular adhesion molecule (ICAM) family. All ICAM proteins are type I transmembrane glycoproteins, contain 2-9 immunoglobulin-like C2-type domains, and bind to the leukocyte adhesion LFA-1 protein. This protein is constitutively and abundantly expressed by all leucocytes and may be the most important ligand for LFA-1 in the initiation of the immune response. It functions not only as an adhesion molecule, but also as a potent signalling molecule. Alternative splicing results in multiple transcript variants encoding different isoforms.

Klonalität:	Monoclonal
Molekulargewicht:	60kDa
NCBI:	3385
UniProt:	P32942
Reinheit:	Affinity purification
Target-Kategorie:	ICAM3

Application Verdünnung:	WB,1:1000 - 1:5000\|IF/ICC,1:200 - 1:800\|IF-P,1:200 - 1:800\|IHC-P,1:200 - 1:2000\|FC,1:500 - 1:1000\|ELISA,Recommended starting concentration is 1 µg/mL. Please optimize the concentration based on your specific assay requirements.
Anwendungsbeschreibung:	Cross-Reactivity: Human. ResearchArea: Signal Transduction,Immunology Inflammation,CDs,Neuroscience,Neurodegenerative Diseases Markers,Other Neurological disorders. Shipping: Ice Bag

	Western blot analysis of various lysates using ICAM3/CD50 Rabbit mAb (A25722)at 1:1600 dilution incubated overnight at 4C. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution. Lysates/proteins: 25µg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020) Negative control (NC): Daudi Exposure time: 1s.
	Immunohistochemistry analysis of paraffin-embedded Human tonsil tissue using ICAM3/CD50 Rabbit mAb (A25722) at a dilution of 1:200 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate buffer (pH 6.0) prior to IHC staining.
	Immunohistochemistry analysis of paraffin-embedded Human spleen tissue using ICAM3/CD50 Rabbit mAb (A25722) at a dilution of 1:200 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate buffer (pH 6.0) prior to IHC staining.
	Confocal imaging of Jurkat cells using ICAM3/CD50 Rabbit mAb (A25722, dilution 1:200) followed by a further incubation with Cy3 Goat Anti-Rabbit IgG (H+L) (AS007, dilution 1:500) (Red). DAPI was used for nuclear staining (Blue). Objective: 100x.
	Confocal imaging of paraffin-embedded Human colon cancer tissue using ICAM3/CD50 Rabbit mAb (A25722, dilution 1:200) followed by a further incubation with Cy3 Goat Anti-Rabbit IgG (H+L) (AS007, dilution 1:500) (Red). DAPI was used for nuclear staining (Blue). High pressure antigen retrieval performed with 0.01M Citrate Buffer (pH 6.0) prior to IF staining. Objective: 40x.
	Confocal imaging of U-937 cells using ICAM3/CD50 Rabbit mAb (A25722, dilution 1:200) followed by a further incubation with Cy3 Goat Anti-Rabbit IgG (H+L) (AS007, dilution 1:500) (Red). DAPI was used for nuclear staining (Blue). Objective: 100x.
	Flow cytometry: 1X10 6 Daudi cells (negative control,left) and U-937 cells (right) were surface-stained with ICAM3/CD50 Rabbit mAb (A25722,2 µg/mL,orange line), or Rabbit IgG isotype control (AC042,2 µg/mL,blue line), followed by Alexa Fluor 647 conjugated goat anti-rabbit pAb staining. Non-fluorescently stained cells were used as blank control (red line).
	Flow cytometry: 1X10 6 U-937 cells were surface-stained with Rabbit IgG isotype control (AC042,2 µg/mL,left) or ICAM3/CD50 Rabbit mAb (A25722,2 µg/mL,right), followed by Alexa Fluor 647 conjugated goat anti-rabbit pAb staining.