[KD Validated] YTHDC1 Rabbit mAb, Unconjugated, Monoclonal
Artikelnummer:
ABB-A22992
- Bilder (8)
| Artikelname: | [KD Validated] YTHDC1 Rabbit mAb, Unconjugated, Monoclonal |
| Artikelnummer: | ABB-A22992 |
| Hersteller Artikelnummer: | A22992 |
| Alternativnummer: | ABB-A22992-100UL,ABB-A22992-500UL,ABB-A22992-20UL,ABB-A22992-1000UL |
| Hersteller: | ABclonal |
| Wirt: | Rabbit |
| Kategorie: | Antikörper |
| Applikation: | ELISA, IHC-P, IP, WB |
| Spezies Reaktivität: | Human |
| Immunogen: | Recombinant protein (or fragment).This information is considered to be commercially sensitive. |
| Konjugation: | Unconjugated |
| Alternative Synonym: | YT521, YT521-B, [KD Validated] YTHDC1 |
| Enables N6-methyladenosine-containing RNA binding activity. Involved in mRNA export from nucleus, mRNA splice site selection, and regulation of gene expression. Located in nuclear speck and plasma membrane. |
| Application Verdünnung: | WB,1:2000 - 1:8000|IHC-P,1:500 - 1:5000|IP,0.5µg-4µg antibody for 200µg-400µg extracts of whole cells|ELISA,Recommended starting concentration is 1 µg/mL. Please optimize the concentration based on your specific assay requirements. |
| Anwendungsbeschreibung: | Cross-Reactivity: Human,Mouse,Rat. ResearchArea: Epigenetics Nuclear Signaling,RNA Binding. Shipping: Ice Bag |
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Western blot analysis of lysates from wild type (WT) and YTHDC1 knockdown (KD) HeLa cells using [KD Validated] YTHDC1 Rabbit mAb (A22992) at 1:3000 dilution incubated overnight at 4°C. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 30s. |
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Immunohistochemistry analysis of paraffin-embeddedMouse colon tissue using[KD Validated] YTHDC1 Rabbit mAb(A22992) at a dilution of 1:1000 (40x lens).High pressure antigen retrieval performed with 0.01M Citrate Buffer (pH 6.0) prior to IHC staining. |
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Western blot analysis of various lysates using [KD Validated] YTHDC1 Rabbit mAb (A22992) at 1:2000 dilution incubated overnight at 4°C. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution. Lysates/proteins: 25µg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 30s. |
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Immunohistochemistry analysis of paraffin-embeddedHuman thyroid cancer tissue using[KD Validated] YTHDC1 Rabbit mAb(A22992) at a dilution of 1:1000 (40x lens).High pressure antigen retrieval performed with 0.01M Citrate Buffer (pH 6.0) prior to IHC staining. |
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Immunohistochemistry analysis of paraffin-embeddedRat colon tissue using[KD Validated] YTHDC1 Rabbit mAb(A22992) at a dilution of 1:1000 (40x lens).High pressure antigen retrieval performed with 0.01M Citrate Buffer (pH 6.0) prior to IHC staining. |
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Immunohistochemistry analysis of paraffin-embeddedHuman small intestine tissue using[KD Validated] YTHDC1 Rabbit mAb(A22992) at a dilution of 1:1000 (40x lens).High pressure antigen retrieval performed with 0.01M Citrate Buffer (pH 6.0) prior to IHC staining. |
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Immunohistochemistry analysis of paraffin-embeddedHuman esophagus tissue using[KD Validated] YTHDC1 Rabbit mAb(A22992) at a dilution of 1:1000 (40x lens).High pressure antigen retrieval performed with 0.01M Citrate Buffer (pH 6.0) prior to IHC staining. |
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Immunohistochemistry analysis of paraffin-embeddedHuman breast cancer tissue using[KD Validated] YTHDC1 Rabbit mAb(A22992) at a dilution of 1:1000 (40x lens).High pressure antigen retrieval performed with 0.01M Citrate Buffer (pH 6.0) prior to IHC staining. |
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