Ube2L3 / UBCH7 Rabbit mAb, Unconjugated, Monoclonal

Artikelname:	Ube2L3 / UBCH7 Rabbit mAb, Unconjugated, Monoclonal
Artikelnummer:	ABB-A20887
Hersteller Artikelnummer:	A20887
Alternativnummer:	ABB-A20887-100UL,ABB-A20887-20UL
Hersteller:	ABclonal
Wirt:	Rabbit
Kategorie:	Antikörper
Applikation:	ELISA, IF, IHC-P, WB
Spezies Reaktivität:	Human
Immunogen:	Synthetic peptide. This information is considered to be commercially sensitive.
Konjugation:	Unconjugated
Alternative Synonym:	E2-F1, L-UBC, UBCH7, UbcM4, Ube2L3 / UBCH7

The modification of proteins with ubiquitin is an important cellular mechanism for targeting abnormal or short-lived proteins for degradation. Ubiquitination involves at least three classes of enzymes: ubiquitin-activating enzymes (E1s), ubiquitin-conjugating enzymes (E2s) and ubiquitin-protein ligases (E3s). This gene encodes a member of the E2 ubiquitin-conjugating enzyme family. This enzyme is demonstrated to participate in the ubiquitination of p53, c-Fos, and the NF-kB precursor p105 in vitro. Several alternatively spliced transcript variants have been found for this gene.

Klonalität:	Monoclonal
Molekulargewicht:	18kDa
NCBI:	7332
UniProt:	P68036
Reinheit:	Affinity purification
Target-Kategorie:	UBE2L3

Application Verdünnung:	WB,1:500 - 1:1000\|IHC-P,1:20 - 1:100\|IF/ICC,1:20 - 1:50\|ELISA,Recommended starting concentration is 1 µg/mL. Please optimize the concentration based on your specific assay requirements.
Anwendungsbeschreibung:	Cross-Reactivity: Human,Mouse,Rat. ResearchArea: Epigenetics Nuclear Signaling,RNA Binding,Cell Biology Developmental Biology,Ubiquitin,Ubiquitin-Proteasome Signaling Pathway,Neuroscience,Neurodegenerative Diseases. Shipping: Ice Bag

	Western blot analysis of various lysates, using Ube2L3 / UBCH7 Rabbit mAb (A20887) at 1:500 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution. Lysates/proteins: 25µg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 3s.
	Immunohistochemistry analysis of paraffin-embedded Human colon carcinoma using Ube2L3 / UBCH7 Rabbit mAb (A20887) at dilution of 1:20 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate buffer (pH 6.0) prior to IHC staining.
	Immunohistochemistry analysis of paraffin-embedded Human placenta using Ube2L3 / UBCH7 Rabbit mAb (A20887) at dilution of 1:20 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate buffer (pH 6.0) prior to IHC staining.
	Immunofluorescence analysis of HeLa cells using Ube2L3 / UBCH7 Rabbit mAb (A20887) at dilution of 1:20 (40x lens). Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.
	Immunofluorescence analysis of K-562 cells using Ube2L3 / UBCH7 Rabbit mAb (A20887) at dilution of 1:20 (40x lens). Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.
	Immunofluorescence analysis of NIH/3T3 cells using Ube2L3 / UBCH7 Rabbit mAb (A20887) at dilution of 1:20 (40x lens). Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.
	Immunofluorescence analysis of PC-12 cells using Ube2L3 / UBCH7 Rabbit mAb (A20887) at dilution of 1:20 (40x lens). Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.
	Immunofluorescence analysis of U2OS cells using Ube2L3 / UBCH7 Rabbit mAb (A20887) at dilution of 1:20 (40x lens). Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.