ASC/TMS1 Rabbit pAb, Unconjugated, Polyclonal
Artikelnummer:
ABB-A16672
- Bilder (7)
| Artikelname: | ASC/TMS1 Rabbit pAb, Unconjugated, Polyclonal |
| Artikelnummer: | ABB-A16672 |
| Hersteller Artikelnummer: | A16672 |
| Alternativnummer: | ABB-A16672-20UL,ABB-A16672-100UL,ABB-A16672-500UL,ABB-A16672-1000UL |
| Hersteller: | ABclonal |
| Wirt: | Rabbit |
| Kategorie: | Antikörper |
| Applikation: | ELISA, IF, IHC-P, IP, WB |
| Spezies Reaktivität: | Human |
| Immunogen: | Recombinant protein (or fragment).This information is considered to be commercially sensitive. |
| Konjugation: | Unconjugated |
| Alternative Synonym: | ASC, TMS, TMS1, CARD5, TMS-1, ASC/TMS1 |
| This gene encodes an adaptor protein that is composed of two protein-protein interaction domains: a N-terminal PYRIN-PAAD-DAPIN domain (PYD) and a C-terminal caspase-recruitment domain (CARD). The PYD and CARD domains are members of the six-helix bundle death domain-fold superfamily that mediates assembly of large signaling complexes in the inflammatory and apoptotic signaling pathways via the activation of caspase. In normal cells, this protein is localized to the cytoplasm, however, in cells undergoing apoptosis, it forms ball-like aggregates near the nuclear periphery. Two transcript variants encoding different isoforms have been found for this gene. |
| Application Verdünnung: | WB,1:1000 - 1:6000|IHC-P,1:50 - 1:200|IF/ICC,1:50 - 1:200|IP,0.5µg-4µg antibody for 400µg-600µg extracts of whole cells|ELISA,Recommended starting concentration is 1 µg/mL. Please optimize the concentration based on your specific assay requirements. |
| Anwendungsbeschreibung: | Cross-Reactivity: Human,Mouse,Rat. ResearchArea: Cancer,Tumor biomarkers,Cell Biology Developmental Biology,Apoptosis,Caspases. Shipping: Ice Bag |
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Immunohistochemistry analysis of paraffin-embedded Human esophageal cancer using ASC/TMS1 Rabbit pAb (A16672) at dilution of 1:200 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate buffer (pH 6.0) prior to IHC staining. |
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Immunohistochemistry analysis of paraffin-embedded Human tonsil using ASC/TMS1 Rabbit pAb (A16672) at dilution of 1:200 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate buffer (pH 6.0) prior to IHC staining. |
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Western blot analysis of various lysates using ASC/TMS1 Rabbit pAb (A16672) at 1:3000 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution. Lysates/proteins: 25µg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 10s. |
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Immunofluorescence analysis of C6 cells using ASC/TMS1 Rabbit pAb (A16672) at dilution of 1:100 (40x lens). Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining. |
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Immunofluorescence analysis of NIH-3T3 cells using ASC/TMS1 Rabbit pAb (A16672) at dilution of 1:100 (40x lens). Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining. |
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Immunofluorescence analysis of U-2 OS cells using ASC/TMS1 Rabbit pAb (A16672) at dilution of 1:100 (40x lens). Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining. |
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Immunoprecipitation analysis of 200 µg extracts of Rat lung using 3 µg ASC/TMS1 antibody (A16672). Western blot was performed from the immunoprecipitate using ASC/TMS1 antibody (A16672) at a dilution of 1:1000. |
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