Nestin Rabbit pAb, Unconjugated, Polyclonal
Artikelnummer:
ABB-A11861
- Bilder (7)
| Artikelname: | Nestin Rabbit pAb, Unconjugated, Polyclonal |
| Artikelnummer: | ABB-A11861 |
| Hersteller Artikelnummer: | A11861 |
| Alternativnummer: | ABB-A11861-20UL,ABB-A11861-100UL,ABB-A11861-1000UL,ABB-A11861-500UL |
| Hersteller: | ABclonal |
| Wirt: | Rabbit |
| Kategorie: | Antikörper |
| Applikation: | ELISA, IF, IHC-P, WB |
| Spezies Reaktivität: | Human |
| Immunogen: | Recombinant protein (or fragment).This information is considered to be commercially sensitive. |
| Konjugation: | Unconjugated |
| Alternative Synonym: | Nbla00170, Nestin |
| This gene encodes a member of the intermediate filament protein family and is expressed primarily in nerve cells. |
| Klonalität: | Polyclonal |
| Molekulargewicht: | 177 kDa |
| NCBI: | 10763 |
| UniProt: | P48681 |
| Reinheit: | Affinity purification |
| Sequenz: | QVPQLLLDPAAWDRDGESDGFADEEESGEEGEEDQEEGREPGAGRWGPGSSVGSLQALSSSQRGEFLESDSVSVSVPWDDSLRGAVAGAPKTALETESQDSAEPSGSEEESDPVSLEREDKVPGPLEIPSGMEDAGPGADIIGVNGQGPNLEGKSQHVNGGVMNGLEQSEEVGQGMPLVSEGDRGSPFQEEEGSALKTSWAGAPVHLGQGQFLKFTQREGDRESWSSGED |
| Target-Kategorie: | NES |
| Antibody Type: | Primary Antibody |
| Application Verdünnung: | WB,1:1000 - 1:3000|IHC-P,1:50 - 1:200|IF/ICC,1:50 - 1:200|ELISA,Recommended starting concentration is 1 µg/mL. Please optimize the concentration based on your specific assay requirements. |
| Anwendungsbeschreibung: | Cross-Reactivity: Human,Mouse,Rat. ResearchArea: Cancer,Tumor biomarkers,Invasion and Metastasis,Cell Biology Developmental Biology,Cell Adhesion,Cytoskeleton,Intermediate Filaments,Neuroscience, Cell Type Marker,Stem Cells,Neural Stem Cells,Cardiovascular,Angiogenesis. Shipping: Ice Bag |
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Immunohistochemistry analysis of paraffin-embedded Mouse brain using Nestin Rabbit pAb (A11861) at dilution of 100 (40x lens). Microwave antigen retrieval performed with 0.01M PBS Buffer (pH 7.2) prior to IHC staining. |
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Immunohistochemistry analysis of paraffin-embedded Rat brain using Nestin Rabbit pAb (A11861) at dilution of 100 (40x lens). Microwave antigen retrieval performed with 0.01M PBS Buffer (pH 7.2) prior to IHC staining. |
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Western blot analysis of lysates from RD cells using Nestin Rabbit pAb (A11861) at 1:3000 dilution incubated overnight at 4°C. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution. Lysates/proteins: 25 µg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 1 s. |
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Immunohistochemistry analysis of paraffin-embeddedHuman thyroid cancer tissue usingNestin Rabbit pAb(A11861) at a dilution of1:1000 (40x lens).High pressure antigen retrieval was performed with 0.01 M citrate buffer (pH 6.0) prior to IHC staining. |
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Immunohistochemistry analysis of paraffin-embeddedHuman spleen tissue usingNestin Rabbit pAb(A11861) at a dilution of1:1000 (40x lens).High pressure antigen retrieval was performed with 0.01 M citrate buffer (pH 6.0) prior to IHC staining. |
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Immunohistochemistry analysis of paraffin-embeddedHuman kidney tissue usingNestin Rabbit pAb(A11861) at a dilution of1:1000 (40x lens).High pressure antigen retrieval was performed with 0.01 M citrate buffer (pH 6.0) prior to IHC staining. |
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Immunofluorescence analysis of Neuro-2a cells using Nestin Rabbit pAb (A11861) at dilution of 1:50 (40x lens). Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining. |
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