Galectin 3/LGALS3 Rabbit mAb, Unconjugated, Monoclonal

Artikelnummer: ABB-A11198
Artikelname: Galectin 3/LGALS3 Rabbit mAb, Unconjugated, Monoclonal
Artikelnummer: ABB-A11198
Hersteller Artikelnummer: A11198
Alternativnummer: ABB-A11198-20UL,ABB-A11198-100UL,ABB-A11198-1000UL,ABB-A11198-500UL
Hersteller: ABclonal
Wirt: Rabbit
Kategorie: Antikörper
Applikation: ELISA, IHC-P, IP, WB
Spezies Reaktivität: Human
Immunogen: Synthetic peptide. This information is considered to be commercially sensitive.
Konjugation: Unconjugated
Alternative Synonym: L31, GAL3, MAC2, CBP35, GALBP, GALIG, LGALS2, Galectin 3/LGALS3
This gene encodes a member of the galectin family of carbohydrate binding proteins. Members of this protein family have an affinity for beta-galactosides. The encoded protein is characterized by an N-terminal proline-rich tandem repeat domain and a single C-terminal carbohydrate recognition domain. This protein can self-associate through the N-terminal domain allowing it to bind to multivalent saccharide ligands. This protein localizes to the extracellular matrix,the cytoplasm and the nucleus. This protein plays a role in numerous cellular functions including apoptosis,innate immunity,cell adhesion and T-cell regulation. The protein exhibits antimicrobial activity against bacteria and fungi. Alternate splicing results in multiple transcript variants.
Klonalität: Monoclonal
Klon-Bezeichnung: [ARC0542]
Molekulargewicht: 26kDa
NCBI: 3958
UniProt: P17931
Reinheit: Affinity purification
Sequenz: MADNFSLHDALSGSGNPNPQGWPGAWGNQPAGAGGYPGASYPGAYPGQAPPGAYPGQAPPGAYPGAPGAYPGAPAPGVYPGPPSGPGAYPSSGQPSATGA
Target-Kategorie: LGALS3
Antibody Type: Primary Antibody
Application Verdünnung: WB,1:1000 - 1:6000|IHC-P,1:200 - 1:800|IP,0.5µg-4µg antibody for 200µg-400µg extracts of whole cells|ELISA,Recommended starting concentration is 1 µg/mL. Please optimize the concentration based on your specific assay requirements.
Anwendungsbeschreibung: Cross-Reactivity: Human,Mouse,Rat. ResearchArea: Epigenetics Nuclear Signaling,RNA Binding,Cell Biology Developmental Biology,Cell Cycle,Cell differentiation,Cell Adhesion,Neuroscience. Shipping: Ice Bag
Immunohistochemistry analysis of paraffin-embedded Human colon tissue using Galectin 3/LGALS3 Rabbit mAb (A11198) at a dilution of 1:500 (40x lens). High pressure antigen retrieval performed with 0.01M Tris-EDTA Buffer (pH 9.0) prior to IHC staining.
Immunohistochemistry analysis of paraffin-embedded Human pancreas tissue using Galectin 3/LGALS3 Rabbit mAb (A11198) at a dilution of 1:500 (40x lens). High pressure antigen retrieval performed with 0.01M Tris-EDTA Buffer (pH 9.0) prior to IHC staining.
Western blot analysis of various lysates using Galectin 3/LGALS3 Rabbit mAb (A11198) at 1:1000 dilution.
Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25µg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 10s.
Immunohistochemistry analysis of paraffin-embedded Human tonsil tissue using Galectin 3/LGALS3 Rabbit mAb (A11198) at a dilution of 1:500 (40x lens). High pressure antigen retrieval performed with 0.01M Tris-EDTA Buffer (pH 9.0) prior to IHC staining.
Immunohistochemistry analysis of paraffin-embedded Rat colon tissue using Galectin 3/LGALS3 Rabbit mAb (A11198) at a dilution of 1:500 (40x lens). High pressure antigen retrieval performed with 0.01M Tris-EDTA Buffer (pH 9.0) prior to IHC staining.
Immunohistochemistry analysis of paraffin-embedded Rat liver tissue using Galectin 3/LGALS3 Rabbit mAb (A11198) at a dilution of 1:500 (40x lens). High pressure antigen retrieval performed with 0.01M Tris-EDTA Buffer (pH 9.0) prior to IHC staining.
Immunohistochemistry analysis of paraffin-embedded Rat spleen tissue using Galectin 3/LGALS3 Rabbit mAb (A11198) at a dilution of 1:500 (40x lens). High pressure antigen retrieval performed with 0.01M Tris-EDTA Buffer (pH 9.0) prior to IHC staining.
Immunoprecipitation analysis of 300 µg extracts of HT-29 cells using 3 µg Galectin 3/LGALS3 antibody (A11198). Western blot was performed from the immunoprecipitate using Galectin 3/LGALS3 antibody (A11198) at a dilution of 1:1000.