ASPN Antibody - middle region

Catalog Number: ASB-ARP42487_T100
Article Name: ASPN Antibody - middle region
Biozol Catalog Number: ASB-ARP42487_T100
Supplier Catalog Number: ARP42487_T100
Alternative Catalog Number: ASB-ARP42487_T100-25UL,ASB-ARP42487_T100-100UL
Manufacturer: Aviva
Host: Rabbit
Category: Proteine/Peptide
Application: IHC, WB
Species Reactivity: Bovine, Canine, Equine, Guinea pig, Human, Mouse, Porcine, Rabbit, Rat, Zebrafish
Immunogen: The immunogen is a synthetic peptide directed towards the middle region of human ASPN
Alternative Names: OS3, PLAP1, PLAP-1, SLRR1C
ASPN belongs to a family of leucine-rich repeat (LRR) proteins associated with the cartilage matrix. The name asporin reflects the unique aspartate-rich N terminus and the overall similarity to decorin.ASPN belongs to a family of leucine-rich repeat (LRR) proteins associated with the cartilage matrix. The name asporin reflects the unique aspartate-rich N terminus and the overall similarity to decorin (MIM 125255) (Lorenzo et al., 2001).[supplied by OMIM].
Clonality: Polyclonal
Concentration: 1.0 mg/ml
Molecular Weight: 43 kDa
NCBI: 54829
UniProt: Q5TBF3
Form: Liquid. Purified antibody supplied in 1x PBS buffer with 0.09% (w/v) sodium azide and 2% sucrose.
25 ug of the indicated Human whole cell extracts was loaded onto a 12% SDS-PAGE gel. 3 ug/mL of the antibody was used in this experiment. ASPN is 43 kDa as a full length protein that is cleaved to ~38 kDa. A smaller isoform of 18-20 kDa is also detected. The mature form is also N-linked glycosylated.
Human Muscle
WB Suggested Anti-ASPN Antibody Titration: 2.5ug/ml
Positive Control: HepG2 cell lysate
Host: Rabbit
Target Name: ASPN
Sample Type: HepG2
Lane A: Primary Antibody
Lane B: Primary Antibody + Blocking Peptide
Primary Antibody Concentration: 2.5ug/mL
Peptide Concentration: 2.0ug/mL
Lysate Quantity: 25ug/lane
Gel Concentration: 12%
Host: Rabbit
Target: ASPN
Positive control (+): Lung tumor (T-LU)
Negative control (-): MCF7 (N10)
Antibody concentration: 1ug/ml
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