TIA1 Antibody - C-terminal region

Article Name:	TIA1 Antibody - C-terminal region
Biozol Catalog Number:	ASB-ARP40981_P050
Supplier Catalog Number:	ARP40981_P050
Alternative Catalog Number:	ASB-ARP40981_P050-25UL,ASB-ARP40981_P050-100UL
Manufacturer:	Aviva
Host:	Rabbit
Category:	Proteine/Peptide
Application:	IP, WB
Species Reactivity:	Bovine, Equine, Guinea pig, Human, Mouse, Rabbit, Rat
Immunogen:	The immunogen is a synthetic peptide directed towards the C terminal region of human TIA1
Alternative Names:	WDM, ALS26, TIA-1

TIA1 is a member of a RNA-binding protein family and possesses nucleolytic activity against cytotoxic lymphocyte (CTL) target cells. It has been suggested that this protein may be involved in the induction of apoptosis as it preferentially recognizes poly(A) homopolymers and induces DNA fragmentation in CTL targets. The major granule-associated species is a 15-kDa protein that is thought to be derived from the carboxyl terminus of the 40-kDa product by proteolytic processing.The product encoded by this gene is a member of a RNA-binding protein family and possesses nucleolytic activity against cytotoxic lymphocyte (CTL) target cells. It has been suggested that this protein may be involved in the induction of apoptosis as it preferentially recognizes poly(A) homopolymers and induces DNA fragmentation in CTL targets. The major granule-associated species is a 15-kDa protein that is thought to be derived from the carboxyl terminus of the 40-kDa product by proteolytic processing. Alternative splicing resulting in different isoforms of this gene product has been described in the literature.

Clonality:	Polyclonal
Concentration:	0.5 mg/ml
Molecular Weight:	42 kDa
NCBI:	7072
UniProt:	P31483-2
Form:	Liquid. Purified antibody supplied in 1x PBS buffer with 0.09% (w/v) sodium azide and 2% sucrose.

	HAP1 WT and TIA1 KO cells were labelled with a green or a far-red fluorescent dye, respectively. WT and KO cells were mixed and plated to a 1:1 ratio in a 96-well plate as a mosaic culture. Cells were stained with ARP40981_P050 at a dilution of 1/500. Bars = 10um.
	TIA1 was immunoprecipitated from 2 mg HEK293 Whole Cell Lysate with ARP40981_P050 with 1:200 dilution. Western blot was performed using ARP40981_P050 at 1/1000 dilution. Lane 1: Control IP in HEK293 Whole Cell Lysate. Lane 2: TIA1 IP with ARP40981_P050 in HEK293 Whole Cell Lysate. Lane 3: Input of HEK293 Whole Cell Lysate.
	WB Suggested Anti-TIA1 Antibody Titration: 0.2-1 ug/ml Positive Control: Human Thymus
	50ug HAP1 WT and TIA1 KO lysates were subjected to SDS-PAGE followed by immunoblotting with an antibody dilution of 1/200. (right) Ponceau S staining to verify protein transfer. Data provided courtesy of the YCharOS Antibody Characterization through Open Science group. Additional data can be found at https://ycharos.com/data
	TIA1 was immunoprecipitated from HAP1 WT cell lysates using 1ug of ARP40981_P050 coupled to Dynabeads. The Ponceau stained transfers of each blot are shown. SM=4% starting material, UB=4% unbound fraction, IP=immunoprecipitated.
	TIA1 antibody - C-terminal region (ARP40981_P050) in Human Thymus using Western Blot