Immunohistochemistry of paraffin-embedded human testis tissue using CSB-PA004862ESR1HU at dilution of 1:100
Immunohistochemistry of paraffin-embedded human colon cancer using CSB-PA004862ESR1HU at dilution of 1:100
Western blot All lanes: CCT5 antibody at 1.78µg/ml Lane 1: Hela whole cell lysate Lane 2: Colo320 whole cell lysate Secondary Goat polyclonal to rabbit IgG at 1/10000 dilution Predicted band size: 60, 50 kDa Observed band size: 60 kDa
Immunoprecipitating CCT5 in Hela whole cell lysate Lane 1: Rabbit control IgG (1µg) instead of CSB-PA004862ESR1HU in Hela whole cell lysate. For western blotting, a HRP-conjugated Protein G antibody was used as the secondary antibody (1/2000) Lane 2: CSB-PA004862ESR1HU (8µg) + Hela whole cell lysate (500µg) Lane 3: Hela whole cell lysate (10µg)
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