CCT5 Antibody, Unconjugated, Rabbit, Polyclonal

Catalog Number: CSB-PA004862ESR1HU
Article Name: CCT5 Antibody, Unconjugated, Rabbit, Polyclonal
Biozol Catalog Number: CSB-PA004862ESR1HU
Supplier Catalog Number: CSB-PA004862ESR1HU
Alternative Catalog Number: CSB-PA004862ESR1HU-100UL, CSB-PA004862ESR1HU-50UL
Manufacturer: Cusabio
Host: Rabbit
Category: Antikörper
Application: ELISA, IHC, IP, WB
Species Reactivity: Human
Conjugation: Unconjugated
Alternative Names: CCT 5 antibody, CCT epsilon antibody, CCT-epsilon antibody, CCT5 antibody, CCTE antibody, CCTepsilon antibody, Chaperonin containing T-complex polypeptide 1, subunit 5 antibody, Chaperonin containing TCP 1 epsilon antibody, Chaperonin containing TCP 1 subunit 5 antibody, Chaperonin containing TCP 1 subunit 5 epsilon antibody, Chaperonin containing TCP1 epsilon antibody, Chaperonin containing TCP1 subunit 5 antibody, Chaperonin containing TCP1 subunit 5 epsilon antibody, KIAA0098 antibody, T complex protein 1 epsilon subunit antibody, T complex protein 1 subunit epsilon antibody, T-complex protein 1 subunit epsilon antibody, TCP 1 epsilon antibody, TCP-1-epsilon antibody, TCP1epsilon antibody, TCPE antibody, TCPE_HUMAN antibody
Clonality: Polyclonal
UniProt: P48643
Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Purity: Antigen Affinity Purified
Form: Liquid
Target: CCT5
Application Dilute: Recommended dilution: WB:1:500-1:5000, IHC:1:20-1:200, IP:1:200-1:2000
Immunohistochemistry of paraffin-embedded human testis tissue using CSB-PA004862ESR1HU at dilution of 1:100
Immunohistochemistry of paraffin-embedded human colon cancer using CSB-PA004862ESR1HU at dilution of 1:100
Western blot
All lanes: CCT5 antibody at 1.78µg/ml
Lane 1: Hela whole cell lysate
Lane 2: Colo320 whole cell lysate
Secondary
Goat polyclonal to rabbit IgG at 1/10000 dilution
Predicted band size: 60, 50 kDa
Observed band size: 60 kDa
Immunoprecipitating CCT5 in Hela whole cell lysate
Lane 1: Rabbit control IgG (1µg) instead of CSB-PA004862ESR1HU in Hela whole cell lysate. For western blotting, a HRP-conjugated Protein G antibody was used as the secondary antibody (1/2000)
Lane 2: CSB-PA004862ESR1HU (8µg) + Hela whole cell lysate (500µg)
Lane 3: Hela whole cell lysate (10µg)