DRAM Antibody, Unconjugated, Rabbit, Polyclonal

Article Name:	DRAM Antibody, Unconjugated, Rabbit, Polyclonal
Biozol Catalog Number:	PRS-4035
Supplier Catalog Number:	4035
Alternative Catalog Number:	PRS-4035-0.02,PRS-4035-0.1
Manufacturer:	ProSci
Host:	Rabbit
Category:	Antikörper
Application:	ELISA, IF, IHC-P, WB
Species Reactivity:	Human, Mouse, Rat
Immunogen:	Anti-DRAM antibody (4035) was raised against a peptide corresponding to 16 amino acids near the amino terminus of human DRAM. The immunogen is located within amino acids 30-80 of DRAM.
Conjugation:	Unconjugated
Alternative Names:	DRAM Antibody: DRAM, DRAM, DNA damage-regulated autophagy modulator protein 1, Damage-regulated autophagy modulator

Clonality:	Polyclonal
Concentration:	1 mg/mL
Molecular Weight:	Predicted: 14kD, 26kDObserved: 14 kD, 26kD
NCBI:	55332
UniProt:	Q8N682
Buffer:	DRAM Antibody is supplied in PBS containing 0.02% sodium azide.
Form:	Liquid

Application Dilute:	Optimal dilutions for each application to be determined by the researcher.
Application Notes:	WB: 1-2 µg/mL, IF: 20 µg/mL, IHC: 2-2.5 µg/mL.Antibody validated: Western Blot in human mouse and rat samples, Immunofluorescence and Immunohistochemistry in human and mouse samples. All other applications and species not yet tested.

	Figure 1 Western Blot Validation in Human 293 Cell LysateLoading: 15 &956,g of lysate per lane.Antibodies: DRAM 4035 (A: 1 &956,g/mL, B: 2 &956,g/mL), 1h incubation at RT in 5% NFDM/TBST.Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10000 dilution.
	Figure 3 Immunofluorescence Validation of DRAM in Human Liver TissueImmunofluorescent analysis of 4% paraformaldehyde-fixed human liver tissue labeling DRAM with 4035 at 20 &956,g/mL, followed by goat anti-rabbit IgG secondary antibody at 1/500 dilution (red).
	Figure 5 Immunofluorescence Validation of DRAM in Mouse Liver TissueImmunofluorescent analysis of 4% paraformaldehyde-fixed mouse liver tissue labeling DRAM with 4035 at 20 &956,g/mL, followed by goat anti-rabbit IgG secondary antibody at 1/500 dilution (red) and DAPI staining (blue).
	Figure 4 Immunohistochemistry Validation of DRAM in Human Liver Tissue Immunohistochemical analysis of paraffin-embedded human liver tissue using anti-DRAM antibody (4035) at 2.5 &956,g/ml. Tissue was fixed with formaldehyde and blocked with 10% serum for 1 h at RT, antigen retrieval was by heat mediation with a citrate buffer (pH6). Samples were incubated with primary antibody overnight at 4&730,C. A goat anti-rabbit IgG H&L (HRP) at 1/250 was used as secondary. Counter stained with Hematoxylin.
	Figure 6 Immunohistochemistry Validation of DRAM in Mouse Liver Tissue Immunohistochemical analysis of paraffin-embedded mouse liver tissue using anti-DRAM antibody (4035) at 2 &956,g/ml. Tissue was fixed with formaldehyde and blocked with 10% serum for 1 h at RT, antigen retrieval was by heat mediation with a citrate buffer (pH6). Samples were incubated with primary antibody overnight at 4&730,C. A goat anti-rabbit IgG H&L (HRP) at 1/250 was used as secondary. Counter stained with Hematoxylin.
	Figure 2 Independent Antibody Validation (IAV) via Protein Expression Profile in Cell LinesLoading: 15 &956,g of lysates per lane.Antibodies: DRAM 4033 (0.5 &956,g/mL), DRAM 4035 (2 &956,g/mL), beta-actin (1 &956,g/mL) and GAPDH (0.02 &956,g/mL), 1h incubation at RT in 5% NFDM/TBST.Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10000 dilution.