RNF43 Antibody - middle region
Artikelnummer:
ASB-ARP43227_P050
- Bilder (6)
| Artikelname: | RNF43 Antibody - middle region |
| Artikelnummer: | ASB-ARP43227_P050 |
| Hersteller Artikelnummer: | ARP43227_P050 |
| Alternativnummer: | ASB-ARP43227_P050-25UL,ASB-ARP43227_P050-100UL |
| Hersteller: | Aviva |
| Wirt: | Rabbit |
| Kategorie: | Proteine/Peptide |
| Applikation: | WB |
| Spezies Reaktivität: | Bovine, Canine, Equine, Human, Mouse, Rabbit, Rat |
| Immunogen: | The immunogen is a synthetic peptide directed towards the middle region of human RNF43 |
| Alternative Synonym: | URCC, SSPCS, RNF124 |
| RNF43 is a HAP95 (AKAP8L) binding ubiquitin ligase that promotes cell growth and is upregulated in colon cancer. |
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WB Suggested Anti-RNF43 Antibody Titration: 0.2-1 ug/ml ELISA Titer: 1:312500 Positive Control: MCF7 cell lysateRNF43 is supported by BioGPS gene expression data to be expressed in MCF7 |
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25 ug of the indicated Human whole cell extracts was loaded onto a 12% SDS-PAGE gel. 1 ug/mL of the antibody was used in this experiment. |
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Host: Rabbit Target Name: RNF43 Sample Tissue: Human Jurkat Whole Cell Antibody Dilution: 1ug/ml |
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Host: Rabbit Target: RNF43 Positive control (+): HepG2 (HG) Negative control (-): A549 (N03) Antibody concentration: 1ug/ml |
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Surface Plasmon Resonance Kinetic Characterization of Polyclonal Antibody Affinity. Purified polyclonal antibodies were immobilized on a Protein A/G coated Carterra LSA sensor chip (PAGH200M) at concentrations of 5, and 50 ug/mL in duplicate. Antibodies on the surface were exposed to interaction with peptides sequentially via microfluidic controlled flow at 333nM peptide concentration for kinetic characterization of the binders for affinity and specificity, followed by curve fitting using the Kinetics software. Kd determinations for both concentrations were averaged and results and standard deviation are shown. |
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