The immunogen is a synthetic peptide directed towards the N terminal region of human SFPQ
Alternative Synonym:
PSF, POMP100, PPP1R140
SFPQ is DNA- and RNA binding protein. It is essential pre-mRNA splicing factor required early in spliceosome formation and for splicing catalytic step II, probably as an heteromer with NONO. It binds to pre-mRNA in spliceosome C complex, and specifically binds to intronic polypyrimidine tracts. It interacts with U5 snRNA. It may be involved in a pre-mRNA coupled splicing and polyadenylation process as component of a snRNP-free complex with SNRPA/U1A. SFPQ may be involved in homologous DNA pairing. SFPQ is involved in transcriptional regulation. It binds the DNA sequence 5-CTGAGTC-3 in the insulin-like growth factor response element (IGFRE) and inhibits IGF-I-stimulated transcriptional activity.
WB Suggested Anti-SFPQ Antibody Titration: 1.25ug/ml ELISA Titer: 1:12500 Positive Control: HepG2 cell lysate.SFPQ is strongly supported by BioGPS gene expression data to be expressed in HepG2
Lanes: Lane 1: 50ug Hela Lysate Primary Antibody Dilution: 1:1000 Secondary Antibody: Anti-rabbit-HRP Secondary Antibody Dilution: 1:10,000 Gene Name: SFPQ Submitted by: Archa Fox, University of Western Australia SFPQ is strongly supported by BioGPS gene expression data to be expressed in Human HeLa cells
SFPQ antibody - N-terminal region (ARP40572_T100) in Human HepG2 using Western Blot
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