TUBA4A Antibody - middle region

Artikelnummer: ASB-ARP40179_P050
Artikelname: TUBA4A Antibody - middle region
Artikelnummer: ASB-ARP40179_P050
Hersteller Artikelnummer: ARP40179_P050
Alternativnummer: ASB-ARP40179_P050-25UL,ASB-ARP40179_P050-100UL
Hersteller: Aviva
Wirt: Rabbit
Kategorie: Proteine/Peptide
Applikation: IHC, IP, WB
Spezies Reaktivität: Bovine, Canine, Equine, Guinea pig, Human, Mouse, Rabbit, Rat, Sheep
Immunogen: The immunogen is a synthetic peptide directed towards the middle region of human TUBA4A
Alternative Synonym: ALS22, TUBA1, H2-ALPHA
Microtubules of the eukaryotic cytoskeleton perform essential and diverse functions and are composed of a heterodimer of alpha and beta tubulin. The genes encoding these microtubule constituents are part of the tubulin superfamily, which is composed of six distinct families. Genes from the alpha, beta and gamma tubulin families are found in all eukaryotes. The alpha and beta tubulins represent the major components of microtubules, while gamma tubulin plays a critical role in the nucleation of microtubule assembly. There are multiple alpha and beta tubulin genes and they are highly conserved among and between species. This gene encodes an alpha tubulin that is a highly conserved homolog of a rat testis-specific alpha tubulin.Microtubules of the eukaryotic cytoskeleton perform essential and diverse functions and are composed of a heterodimer of alpha and beta tubulin. The genes encoding these microtubule constituents are part of the tubulin superfamily, which is composed of six distinct families. Genes from the alpha, beta and gamma tubulin families are found in all eukaryotes. The alpha and beta tubulins represent the major components of microtubules, while gamma tubulin plays a critical role in the nucleation of microtubule assembly. There are multiple alpha and beta tubulin genes and they are highly conserved among and between species. This gene encodes an alpha tubulin that is a highly conserved homolog of a rat testis-specific alpha tubulin. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Entrez Gene record to access additional publications.
Klonalität: Polyclonal
Konzentration: 0.5 mg/ml
Molekulargewicht: 50 kDa
NCBI: 7277
UniProt: P68366
Formulierung: Liquid. Purified antibody supplied in 1x PBS buffer with 0.09% (w/v) sodium azide and 2% sucrose.
Sample Type:
Mouse brain stem cellsPrimary Antibody Dilution:
1:500Secondary Antibody:
Goat anti-rabbit Alexa-Fluor 594Secondary Antibody Dilution:
1:1000Color/Signal Descriptions:
TUBA4A: Red DAPI:BlueGene Name:
TUBA4A Submitted by:
Dr. Yuzhi Chen, University of Arkansas for Medical Science
Amount and Sample Type:
500 ug mouse brain homogenate
Amount of IP Antibody:
6 ug
Primary Antibody:
TUBA4A
Primary Antibody Dilution:
1:500
Secondary Antibody:
Goat anti-rabbit Alexa-Fluor 594
Secondary Antibody Dilution:
1:5000
Gene Name:
TUBA4A
Submitted by:
Dr. Yuzhi Chen, University of Arkansas for Medical Science
WB Suggested Anti-TUBA4A Antibody Titration: 0.2-1 ug/ml
ELISA Titer: 1:12500
Positive Control: Human Placenta
Sample Type: 1. Human NT-2 cells (60ug)
2. and 3. mouse brain extracts (80ug)
4. rat brain extractA(80ug)
Primary Antibody Dilution:A2ug/ml
Secondary Antibody: IRDye 800CW goat anti-rabbit from Li-COR Bioscience
Secondary Antibody Dilution:A1: 20,000
Image Submitted by: Yuzhi Chen
University of Arkansas for Medical ScienceA
25 ug of the indicated Human whole cell extracts was loaded onto a 12% SDS-PAGE gel. 1 ug/mL of the antibody was used in this experiment. Canonical 50 kDa isoform is identified, and a second isoform of 48 kDa is also present in some samples.
TUBA4A antibody - middle region (ARP40179_P050) in Human Placenta using Western Blot