RPL13 Antibody, Unconjugated, Rabbit, Polyclonal

Artikelnummer: PRS-22-437
Artikelname: RPL13 Antibody, Unconjugated, Rabbit, Polyclonal
Artikelnummer: PRS-22-437
Hersteller Artikelnummer: 22-437
Alternativnummer: PRS-22-437-100
Hersteller: ProSci
Wirt: Rabbit
Kategorie: Antikörper
Applikation: IF, IHC, WB
Spezies Reaktivität: Human, Mouse, Rat
Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 1-211 of human RPL13 (NP_150254.1).
Konjugation: Unconjugated
Alternative Synonym: RPL13, BBC1, D16S444E, FLJ27453, FLJ27454, MGC117342, MGC71373, L13, D16S44E
Klonalität: Polyclonal
Konzentration: batch dependent
Molekulargewicht: Observed: 24kDa
NCBI: 6137
UniProt: P26373
Puffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Formulierung: Liquid
Application Verdünnung: Optimal dilutions for each application to be determined by the researcher.
Anwendungsbeschreibung: WB: 1:500 - 1:2000IHC: 1:50 - 1:200IF: 1:50 - 1:200
Western blot analysis of extracts of various cell lines, using RPL13 antibody (22-437) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit.
Exposure time: 1s.
Immunohistochemistry of paraffin-embedded rat spinal cord using RPL13 Antibody (22-437) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded rat brain using RPL13 Antibody (22-437) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded human stomach using RPL13 Antibody (22-437) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded human gastric cancer using RPL13 Antibody (22-437) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded mouse brain using RPL13 Antibody (22-437) at dilution of 1:100 (40x lens).
Immunofluorescence analysis of MCF-7 cells using RPL13 antibody (22-437). Blue: DAPI for nuclear staining.